Abstract: The response surface method was used to optimize the ultrasound-assisted enzymatic extraction process of Cordyceps militaris., and the resultant extract was tested for hypoglycemic activity, so as to provide technical support for the comprehensive development and utilization of C. militaris resources. The effects of pH, cellulase dosage, enzymolysis time and temperature of ultrasound-treatment on the yield of cordycepin were investigated through single factor experiments to determine the optimal extraction process. The insulin resistance(IR)model of HepG2 cells was established to study the effect of concentration and time-dependent of cordycepin on hypoglycemic activity. The results showed that the best extraction process for cordycepin was: cellulase dosage 1.60% (m/m), enzymolysis time 60 min, temperature of ultrasound-treatment 55 ℃, and ultrasonic power 400 W. Under this condition, the yield of cordycepin was (8.097±0.028) mg/g. The best insulin resistance model was induced by 1.0 μmol/L insulin for 12 h. The highest glucose consumption was (4.554±0.008) mmol/L in 24 h with 0.25 g/L cordycepin. In conclusion, the ultrasonic-assisted enzymatic hydrolysis method can efficiently extract cordycepin, and the extract can improve the insulin resistance of HepG2 cells.
符群,郐滨,钟明旭,等. 超声波辅助酶解法提取北虫草菌素及其降血糖活性研究[J]. 食品与发酵工业, 2021, 47(9): 120-127.
FU Qun,KUAI Bin,ZHONG Mingxu,et al. Extraction of cordycepin with ultrasound-assisted enzymatic hydrolysis and its hypoglycemic activity[J]. Food and Fermentation Industries, 2021, 47(9): 120-127.
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