Abstract: Physalis alkekengi L. is rich in bioactive phytochemicals with a wide of range of biological activities and health benefits. Calyx of physalis (Cps) is the major plant part used for the treatment of sore throat, cough, eczema, hepatitis, urinary problems, and tumors. Over the past 20 years, many scientists extensively investigated the active components of calyx, such as physalins, alkaloids, flavonoids, saponins. However, little is known about their effects on anti-aging. To explore the effect of Cps on the lifespan of Caenorhabditis elegans and its mechanism, oxidative stress model was constructed using Juglone as the oxidant. The survival ability of wild-type (N2) and mutant worms (eat-2、daf-2、clk-1) and the motility, reproduction ability and lifespan of wild-type (N2) worms after Cps treatment were investigated. The expression of related antiaging genes was detected by qPCR. Results showed that supplementation with Cps improved the anti-oxidative stress ability of N2、eat-2、daf-2 worms (P<0.01or P<0.05), improved the motility and reproduction ability (P<0.05) of N2 worms. However, Cps had no significant effect on the antioxidant stress ability and lifespan of eat-2 mutant. Meanwhile, treatment with Cps could increase the transcription level of antioxidant related genes, including daf-16, gst-4, sod-3 and hsp-16.2 (P<0.01). The Cps has significant anti-oxidant biological activity in vivo, and can delay the aging of worms. The mechanism may be by activating the dietary restriction signal pathway, up-regulate related antioxidant genes, enhancing the anti-oxidative stress ability of worms, and delaying the aging of worms.
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