Screening and application of biogenic amines degrading strain derived from Luzhou-flavor Daqu
ZENG Yuxue1, LUO Huibo1,3, YU Dong2, HUANG Dan1,3, GUO Huixiang2, ZOU Yongfang2*
1(College of Bioengineering,Sichuan University of Science ＆ Engineering,Yibin 644000,China) 2(China Ecological Brewing Industry Technology Research Institute,Shede Winery Co., Ltd.,Suining 629200,China) 3(Liquor Making Bio-Technology ＆ Application of Key Laboratory of Sichuan Province,Yibin 644000,China)
Abstract: Biogenic amines degrading strains were screened from Luzhou-flavor liquor Daqu, and their growth characteristics and ability to degrade biogenic amines under solid-state fermentation conditions were studied. The content of biogenic amines in wheat extract was determined by high performance liquid chromatography, and a yeast strain with the best degradation effect was obtained. It was identified as Kodamaea ohmeri by morphology and molecular biology, and named Kodamaea ohmeri HJM. The growth characteristics of K. ohmeri HJM were studied. The highest tolerance temperature was 43 ℃, the highest ethanol tolerance concentration was 14%, the lowest acid tolerance was pH 2, and it exhibited high glucose tolerance. Under the condition of solid-state fermentation, there was significant difference in biogenic amines content between the inoculated liquid and the blank control group (P< 0.05). The degradation rates of biogenic amines were: putrescine (39.19±0.08)%, cadaverine (33.77±0.06)%, methylamine (32.44±0.06)%, ethylamine (23.39±0.06)%, pyrrolidine (63.42±0.02)%, isoamylamine (49.83 ± 0.07)%, cyclohexylamine (49.73±0.03)%, cyclopentamine (66.07±0.08)%. In this study, strains with high biodegradation rate of biogenic amines were isolated from Luzhou-flavor Daqu, which could provide reference for the regulation of biogenic amines in Baijiu.
LINARES D M, DEL-RI'O B, LADERO V, et al.Factors influencing biogenic amines accumulation in dairy products[J].Frontiers in Microbiology, 2012,3(180):180.
[2]
王春利, 赵晓娟, 王英姿.白酒中8种生物胺的高效液相色谱分析法[J].酿酒科技, 2017,(11):113-116.WANG C L, ZHAO X J, WANG Y Z.Determination of 8 biogenic amines in baijiu by HPLC[J].Wine Science and Technology, 2017(11):113-116.
[3]
WUNDERLICHOVA L,BUNKOVA L,KOUTNY M, et al.Formation, degradation, and detoxification of putrescine by foodborne bacteria:A review[J].Comprehensive Reviews in Food Science & Food Safety, 2015,13:1 012-1033.
[4]
王挥, 付湘晋, 吴伟, 等.1株奥默柯达酵母对鱼露中生物胺的降解特性[J].中国食品学报, 2014,14(8):137-141.WANG H, FU X J, WU W, et al.Degradation characteristics of biogenic amine in fish sauce by a strain of Kodamaea ohmeri[J].Chinese Journal of Food Science, 2014,14(8):137-141.
[5]
刘景, 任婧, 孙克杰.食品中生物胺的安全性研究进展[J].食品科学, 2013,34(5):322-326.LIU J, REN J, SUN K J.Safety of biogenic amines in foods[J].Food Science, 2013,34(5):322-326.
[6]
曾雪晴, 李洪军, 袁琳娜, 等.郫县豆瓣酱中生物胺含量和种类分析[J].食品与发酵工业, 2019,45(19):258-265.ZENG X Q, LI H J, YUAN L N,et al.Types and contents of biogenic amines in Pixian horsebean paste[J].Food and Fermentation Industries, 2019,45(19):258-265.
[7]
MAJCHERCZYK J, SURÓWKA K.Effects of onion or caraway on the formation of biogenic amines during sauerkraut fermentation and refrigerated storage[J].Food Chemistry, 2019,298:125 083.
[8]
SHUKLA S, LEE J S, BAJPAI V K, et al.Toxicological evaluation of lotus, ginkgo, and garlic tailored fermented Korean soybean paste (Doenjang) for biogenic amines, aflatoxins, and microbial hazards[J].Food and Chemical Toxicology, 2019,133:110 729.
[9]
DONG H, XIAO K J.Modified QuEChERS combined with ultra high performance liquid chromatography tandem mass spectrometry to determine seven biogenic amines in Chinese traditional condiment soy sauce[J].Food Chemistry, 2017,229:502-508.
[10]
JIA W, FAN Z B, DU A, et al.Recent advances in Baijiu analysis by chromatography based technology-A review[J].Food chemistry, 2020,324:126 899.
[11]
杜木英, 陈宗道, 阚建全, 等.青稞酒发酵过程中生物胺动态变化[J].食品科学, 2012,33(3):163-167.DU M Y, CHEN Z D, KAN J Q, et al.Dynamic changes of biogenic amines in highland barley wine during fermentation[J].Food Science, 2012,33(3):163-167.
[12]
温永柱, 范文来, 徐岩.GC-MS法定性白酒中的多种生物胺[J].酿酒, 2013,40(1):38-41.WEN Y Z, FAN W L, XU Y.Identification of a variety of biogenic amines by GC-MS method in Chinese liquor[J].Winemaking, 2013,40(1):38-41.
[13]
温永柱, 范文来, 徐岩, 等.白酒中5种生物胺的HPLC定量分析[J].食品工业科技, 2013,34(7):305-308.WEN Y Z, FAN W L, XU Y, et al.Quantification for 5 selected biogenic amines in Chinese liquor by HPLC[J].Science and Technology of Food Industry, 2013,34(7):305-308.
[14]
范文来, 徐岩, 温永柱.白酒发酵与蒸馏过程中5种生物胺变化[J].食品工业科技, 2015,36(9):144-146.FAN W L, XU Y, WEN Y Z.Quantification for 5 selected biogenic amines in fermentation and distillation of Chinese liquor by HPLC[J].Science and Technology of Food Industry, 2015,36(9):144-146.
[15]
王涛, 张文学, 邹强, 等.我国蒸馏白酒中内生性风险物质现状浅析[J].中国酿造, 2019,38(5):13-17.WANG T, ZHANG W X, ZOU Q, et al.Brief analysis of endogenous risk substances in distilled Baijiu in China[J].China Brewing, 2019,38(5):13-17.
[16]
唐垚,唐小曼,明建英,等.四川泡菜产生物胺细菌的筛选及产胺能力验证[J].中国调味品, 2019,44(7):81-84.TANG Y, TANG X M, MING J Y, et al.Screening of amine-producing bacteria from Sichuan pickle and verification of amine-producing ability[J].China Seasoning, 2019,44(7):81-84.
[17]
谷静思. 传统发酵臭豆腐中微生物菌相结构解析及其与生物胺形成的关系[D].杭州:浙江大学, 2018.GU J S.Biodiversity of Chinese traditional stinky tofu microbiota and its biogenic amines metabolism[D].Hangzhou:Zhejiang University, 2018.
[18]
赵佳迪, 单万祥, 钮成拓, 等.豆瓣酱生物胺降解菌株的筛选、鉴定及其降解特性研究[J].食品与发酵工业, 2020,46(9):64-72.ZHAO J D, SHAN W X, NIU C T, et al.Screening, identification and biogenic amines-degrading bacteria from Doubanjiang[J].Food and Fermentation Industries, 2020,46(9):64-72.
[19]
牛天娇, 陈历水, 孔杭如, 等.传统发酵肉制品中降解生物胺菌株的筛选鉴定与应用研究[J].中国酿造, 2019,38(9):43-48.NIU T J, CHEN L S, KONG H R, et al.Screening, identification and application of biogenic amine degrading strains derived from traditional fermented meat products[J].China Brewing, 2019,38(9):43-48.
[20]
中华人民共和国国家卫生和计划生育委员会, 国家食品药品监督管理总局.GB 5009.208—2016, 食品安全国家标准 食品中生物胺的测定[S].北京:中国标准出版社, 2016.National Health and Family Planning Commission of the People's Republic of China, State Food and Drug Administration.GB 5009.208—2016, National food safety standard determination of biogenic amines in food[S].Beijing:China Standard Press, 2016.
[21]
FRI'AS J, MARTI'NEZ-VILLALUENGA C, GUILEWICZ P, et al.Biogenic amines and HL60 citotoxicity of alfalfa and fenugreek sprouts[J].Food Chemistry, 2007,105(3):959-967
[22]
陈智毅, 陈聪汉, 肖永坚, 等.高效液相色谱法测定米酒中生物胺的含量[J].食品科学, 2013(12):229-233.CHEN Z Y, CHEN C H, XIAO Y J, et al.Determination of biogenic amines in rice wine by high performance liquid chromatography[J].Food Science, 2013(12):229-233.
[23]
BARNETT J A, PAYNE R W, YARROW D, 等.酵母菌的特征与鉴定手册[M].青岛:海洋大学出版社, 1991:414-430.BARNETT J A, PAYNE R W, YARROW D, et al.Handbook of Characteristics and Identification of Yeast[M].Qingdao:Ocean University Press, 1991:414-430.
[24]
ABOUL-MAATY A F, ORABY A S.Extraction of high-quality genomic DNA from different plant orders applying a modified CTAB- based method[J/OL].Bulletin of the National Research Centre, 2019.DOI:10.1186/S42269-019-0066-1.
[25]
张芸曌. 中高温大曲主发酵期微生物群落与环境因子及理化性质的关联性研究[D].自贡:四川轻化工大学, 2019.ZHANG Y Z.Study on the correlation between microbial community and environmental factors and physicochemical properties during the main fermentation period of medium-high temperature Daqu[D].Zigong:Sichuan University of Science& Engineering, 2019.
[26]
邓丽丽, 王佳文, 袁丁, 等.竹节参总皂苷通过NLRP1和NLRP3炎症小体途径减轻衰老大鼠神经细胞凋亡的作用研究[J].中草药, 2017, 48(23):4 941-4 951.DENG L L, WANG J W, YUAN D, et al.Effect of saponins from Panax japonicus on neuronal apoptosis of natural aging rats by NLRP1 and NLRP3 inflammasome pathway[J].Chinese Traditional and Herbal Drugs, 2017, 48(23):4 941-4 951.
2021, Vol. 47 
