Screening and application of biogenic amines degrading strain derived from Luzhou-flavor Daqu
ZENG Yuxue1, LUO Huibo1,3, YU Dong2, HUANG Dan1,3, GUO Huixiang2, ZOU Yongfang2*
1(College of Bioengineering,Sichuan University of Science & Engineering,Yibin 644000,China) 2(China Ecological Brewing Industry Technology Research Institute,Shede Winery Co., Ltd.,Suining 629200,China) 3(Liquor Making Bio-Technology & Application of Key Laboratory of Sichuan Province,Yibin 644000,China)
Abstract: Biogenic amines degrading strains were screened from Luzhou-flavor liquor Daqu, and their growth characteristics and ability to degrade biogenic amines under solid-state fermentation conditions were studied. The content of biogenic amines in wheat extract was determined by high performance liquid chromatography, and a yeast strain with the best degradation effect was obtained. It was identified as Kodamaea ohmeri by morphology and molecular biology, and named Kodamaea ohmeri HJM. The growth characteristics of K. ohmeri HJM were studied. The highest tolerance temperature was 43 ℃, the highest ethanol tolerance concentration was 14%, the lowest acid tolerance was pH 2, and it exhibited high glucose tolerance. Under the condition of solid-state fermentation, there was significant difference in biogenic amines content between the inoculated liquid and the blank control group (P< 0.05). The degradation rates of biogenic amines were: putrescine (39.19±0.08)%, cadaverine (33.77±0.06)%, methylamine (32.44±0.06)%, ethylamine (23.39±0.06)%, pyrrolidine (63.42±0.02)%, isoamylamine (49.83 ± 0.07)%, cyclohexylamine (49.73±0.03)%, cyclopentamine (66.07±0.08)%. In this study, strains with high biodegradation rate of biogenic amines were isolated from Luzhou-flavor Daqu, which could provide reference for the regulation of biogenic amines in Baijiu.
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