Abstract: In this paper, a Ni/Co layered double hydroxides based oxidase-mimicking enzyme sensor was established for the colorimetric detection of Hg2+ in seafood. The Ni/Co layered double metal hydroxide (Ni/Co LDHs) with oxidase-like activity was prepared by co-precipitation method, and characterized by SEM, XRD and FTIR. The results showed that the Ni/Co LDHs can oxidize 3, 3', 5, 5'-tetramethylbenzidine (TMB) into oxTMB with color change into blue. The medium pH, TMB concentration, Ni/Co LDHs concentration, incubation time and temperature were optimized to obtain the optimal parameters for the detection of Hg2+, which were pH of 4.0, TMB concentration of 0.5 mmol/L, incubation temperature of 25 ℃, incubation time of 10 min, and Ni/Co LDHs concentration of 0.8 mg/mL. The corresponding standard curve was established under the optimal detection conditions, and the sensor showed a linear response in the range of 2.96-11.63 μmol/L with the limit of detection (LOD) of 30.60 nmol/L. The recoveries of spiked samples were 92.74%-114.40%. This study can provide a new idea for the detection of Hg2+ in seafood.
侯菊英. 基于碳量子点荧光技术快速测定果汁中的汞离子和有机磷农药残留[D].泰安:山东农业大学, 2015.HOU J Y.Rapid detection of Hg2+ and organophosphorus pesticides in fruit juices based on carbon dots and fluorescent technique[D].Taian:Shandong Agricultural University, 2015.
[2]
张家一. 农业用水中汞离子检测的电化学发光生物传感器研究[D].镇江:江苏大学, 2019.ZHANG J Y.Study on electrochemiluminescence biosensor for determination of mercury ion in agricultural water[D].Zhenjiang:Jiangsu University, 2019.
[3]
CHRISTUS A A B, RAVIKUMAR A, PANNEERSELVAM P, et al.A novel Hg(II) sensor based on Fe3O4@ZnO nanocomposite as peroxidase mimics[J].Applied Surface Science, 2018, 449:669-676.
[4]
MATOS R A M, GOMES T S R M, OLIVEIRA A B P J, et al.Assessment of transition metals toxicity in environmental matrices using potentiometric electrodes:Inorganic mercury(II) in the seawater as a case study[J].Electroanalysis, 2015, 27(8):1 932-1 938.
[5]
赵明岩. 水中汞的测定方法对比研究[J].资源节约与环保, 2013, 32(12):94.ZHAO M Y.Comparative study on determination methods of mercury in water[J].Resources Economization and Environmental Protection, 2013, 32(12):94.
[6]
李祎萌. 食品汞含量分析预处理与测定技术的研究进展[J].广东化工, 2018, 45(24):33-34.LI Y M.The research progress of determination method of mercury in food[J].Guangdong Chemical Industry, 2018, 45(24):33-34.
[7]
ZHU S, CHEN B, HE M, et al.Speciation of mercury in water and fish samples by HPLC-ICP-MS after magnetic solid phase extraction[J].Talanta, 2017, 171:213-219.
[8]
付瑜. 石墨烯量子点在水溶液中对汞离子、碘离子和有机小分子的检测研究[D].上海:上海师范大学, 2017.FU Y.Graphene quantum dots for the detection of mercury, iodine, organic molecules in aqueous solution[D].Shanghai:Shanghai Normal University, 2017.
[9]
CHRISTUS A A B, PANNEERSELVAM P, RAVIKUMAR A, et al.Colorimetric determination of Hg(II) sensor based on magnetic nanocomposite (Fe3O4@ZIF-67) acting as peroxidase mimics[J].Journal of Photochemistry and Photobiology A:Chemistry, 2018, 364:715-724.
[10]
XIONG Y, SU L, YANG H, et al.Fabrication of copper sulfide using a Cu-based metal organic framework for the colorimetric determination and the efficient removal of Hg2+ in aqueous solutions[J].New Journal of Chemistry, 2015, 39(12):9 221-9 227.
[11]
KONGASSERI A, SOMPALLI N K, RAO B C V S, et al.Solid-state optical sensing of ultra-trace Hg2+ ions using chromoionophoric probe anchored silica monolithic architectures[J].Sensors and Actuators B:Chemical, 2020:321:128 558.
[12]
WU C, GAO G Z, ZHAI K F, et al.A visual Hg2+ detection strategy based on distance as readout by G-quadruplex DNAzyme on microfluidic paper[J].Food Chemistry, 2020:331:127 208.
[13]
ZHANG S, WEI S L, CHENG H, et al.A highly sensitive colorimetric sensor for Hg2+ detection based on the oxidative enzyme mimics-like activity of hierarchical porous carbon@chitosan-modified silver nanoparticles[J].Journal of King Saud University-Science, 2020, 32(2):1 265-1 271.
[14]
YU R Z, WANG R, HE X, et al.Simulated enzyme inhibition-based strategy for ultrasensitive colorimetric biothiol detection based on nanoperoxidases[J].Chemical Communications, 2019, 55(77):11 543-11 546.
[15]
SHARMA V, SAINI A K, MOBIN S M.Multicolor fluorescent carbon nanoparticle probes for live cell imaging and dual palladium and mercury sensors[J].Journal of Materials Chemistry B, 2016, 4(14):2 466-2 476.
[16]
XIE Z J, BAO X Y, PENG C F.Highly sensitive and selective colorimetric detection of methylmercury based on DNA functionalized gold nanoparticles[J].Sensors, 2018, 18(8):2 679.
[17]
ZHANG Y, JU P, SUN L P, et al.Colorimetric determination of Hg2+ based on the mercury-stimulated oxidase mimetic activity of Ag3PO4 microcubes[J].Microchimica Acta, 2020, 187(7):422.
[18]
LI W, CHEN B, ZHANG H X, et al.BSA-stabilized Pt nanozyme for peroxidase mimetics and its application on colorimetric detection of mercury(II) ions[J].Biosensors and Bioelectronics, 2015, 66:251-258.
[19]
LIAN Q, LIU H, ZHENG X F, et al.Enhanced peroxidase-like activity of CuO/Pt nanoflowers for colorimetric and ultrasensitive Hg2+ detection in water sample[J].Applied Surface Science, 2019, 483:551-561.
[20]
YIN J F, CAO H Q, LU Y X.Self-assembly into magnetic Co3O4 complex nanostructures as peroxidase[J].Journal of Materials Chemistry, 2012, 22(2):527-534.
[21]
YANG H G, XIONG Y H, ZHANG P, et al.Colorimetric detection of mercury ions using MnO2 nanorods as enzyme mimics[J].Analytical Methods, 2015, 7(11):4 596-4 601.
[22]
雷立旭, 张卫锋, 胡猛, 等.层状复合金属氢氧化物:结构、性质及其应用[J].无机化学学报, 2005, 21(4):452-460.LEI L X, ZHANG W F, HU M, et al.Layered double hydroxides:Structures, properties and applications[J].Chinese Journal of Inorganic Chemistry, 2005, 21(4):452-460.
[23]
FAN G, WANG H, XIANG X, et al.Co-Al mixed metal oxides/carbon nanotubes nanocomposite prepared via a precursor route and enhanced catalytic property[J].Journal of Solid State Chemistry, 2013, 197:14-22.
[24]
BAI Z M, WANG Z Y, ZHANG T G, et al.Characterization and friction performances of Co-Al-layered double-metal hydroxides synthesized in the presence of dodecyl sulfate[J].Applied Clay Science, 2013, 75-76:22-27.
[25]
GENTY E, BRUNET J, POUPIN C, et al.Co-Al mixed oxides prepared via LDH route using microwaves or ultrasound:Application for catalytic toluene total oxidation[J].Catalysts, 2015, 5(2):851-867.
[26]
周洁. 分级多孔层状双金属氢氧化物的水热合成及吸附性能[D].武汉:武汉理工大学, 2015.ZHOU J.Hierarchically porous layered double hydroxides hydrothermal synthesis and adsorption performance[D].Wuhan:Wuhan University of Technology, 2015.
[27]
LAN L, NIU Q, LI T.A highly selective colorimetric and ratiometric fluorescent probe for instantaneous sensing of Hg2+in water, soil and seafood and its application on test strips[J].Analytica Chimica Acta, 2018, 1 023:105-114.
[28]
DONG T, ZHANG X, LI M, et al.Hierarchical flower-like Ni-Co layered double hydroxide nanostructures:Synthesis and super performance[J].Inorganic Chemistry Frontiers, 2018, 5(12):3 033-3 041.
[29]
SU L, YU X, QIN W, et al.One-step analysis of glucose and acetylcholine in water based on the intrinsic peroxidase-like activity of Ni/Co LDHs microspheres[J].Journal of Materials Chemistry B, 2017, 5(1):116-122.