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食品与发酵工业  2021, Vol. 47 Issue (8): 204-211    DOI: 10.13995/j.cnki.11-1802/ts.025767
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
Ni/Co层状氢氧化物模拟氧化物酶可视化检测海产品中Hg2+
赵海萍, 南丽娟, 雒雪丽, 杨伟霞, 韩雍, 李忠宏*
(西北农林科技大学 食品科学与工程学院,陕西 杨凌,712100)
Ni/Co layered hydroxide based oxidase-mimicking enzyme for colorimetric detection of mercury (Ⅱ) in seafood
ZHAO Haiping, NAN Lijuan, LUO Xueli, YANG Weixia, HAN Yong, LI Zhonghong*
(Northwest A&F University, College of Food Science and Engineering, Yangling 712100, China)
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摘要 该文建立了一种基于Ni/Co层状氢氧化物模拟氧化物酶的传感器比色检测海产品中Hg2+的方法。通过共沉淀法制备了具有氧化物酶活性的Ni/Co层状双金属氢氧化物(layered double hydroxides, LDHs),并对其表面形貌、晶体结构和表面基团进行表征。结果表明,Ni/Co LDHs可使3,3',5,5'-四甲基联苯胺(3,3',5,5'-tetramethylbenzidine, TMB)氧化为蓝色的oxTMB;以检测体系pH、TMB浓度、Ni/Co LDHs浓度、孵育时间、孵育温度为单因素,优化得到检测Hg2+的最佳条件为pH 4.0、TMB浓度为 0.5 mmol/L、孵育温度为25 ℃、孵育时间为10 min、Ni/Co LDHs质量浓度为0.8 mg/mL。在最优检测条件下建立了标准曲线,该传感器在2.96~11.63 μmol/L具有线性响应,检测限为30.60 nmol/L,加标回收率为92.74%~114.40%。该研究为海产品中Hg2+ 的检测提供了一种新思路。
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赵海萍
南丽娟
雒雪丽
杨伟霞
韩雍
李忠宏
关键词:  层状氢氧化物  重金属  纳米酶  可视化检测  海产品    
Abstract: In this paper, a Ni/Co layered double hydroxides based oxidase-mimicking enzyme sensor was established for the colorimetric detection of Hg2+ in seafood. The Ni/Co layered double metal hydroxide (Ni/Co LDHs) with oxidase-like activity was prepared by co-precipitation method, and characterized by SEM, XRD and FTIR. The results showed that the Ni/Co LDHs can oxidize 3, 3', 5, 5'-tetramethylbenzidine (TMB) into oxTMB with color change into blue. The medium pH, TMB concentration, Ni/Co LDHs concentration, incubation time and temperature were optimized to obtain the optimal parameters for the detection of Hg2+, which were pH of 4.0, TMB concentration of 0.5 mmol/L, incubation temperature of 25 ℃, incubation time of 10 min, and Ni/Co LDHs concentration of 0.8 mg/mL. The corresponding standard curve was established under the optimal detection conditions, and the sensor showed a linear response in the range of 2.96-11.63 μmol/L with the limit of detection (LOD) of 30.60 nmol/L. The recoveries of spiked samples were 92.74%-114.40%. This study can provide a new idea for the detection of Hg2+ in seafood.
Key words:  layered double hydroxide    heavy metals    nanozyme    visual detection    seafood
               出版日期:  2021-04-25      发布日期:  2021-05-20      期的出版日期:  2021-04-25
基金资助: 国家自然科学基金项目(32060571;31801628);中央政府指导地方科学技术发展的专项规划项目(2019QNKB2-4)
作者简介:  硕士研究生(李忠宏教授为通讯作者,E-mail:lizhhsteve@163.com)
引用本文:    
赵海萍,南丽娟,雒雪丽,等. Ni/Co层状氢氧化物模拟氧化物酶可视化检测海产品中Hg2+[J]. 食品与发酵工业, 2021, 47(8): 204-211.
ZHAO Haiping,NAN Lijuan,LUO Xueli,et al. Ni/Co layered hydroxide based oxidase-mimicking enzyme for colorimetric detection of mercury (Ⅱ) in seafood[J]. Food and Fermentation Industries, 2021, 47(8): 204-211.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025767  或          http://sf1970.cnif.cn/CN/Y2021/V47/I8/204
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