Acceleration of sardine fish sauce fermentation using exogenous
ZHAO Shuaidong1, LIU Ting1, JI Xu1, YANG Zilu1, YIN Xuanwei1, SHI Wenzheng1, WANG Liping1,2,3*, NING Xibin1*
1(School of Food Science and Technology, Shanghai Ocean University, Shanghai 210306, China) 2(Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China) 3(Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai 201306, China)
Abstract: This study aimed to shorten the fermentation process of sardine fish sauce using exogenous protease and Aspergillus YL001. Sardine was divided into two groups, one fermented with exogenous protease only (fish sauce A) and the other fermented with exogenous protease and Aspergillus YL001 (fish sauce B). Fermentation was carried out at 35℃ for 30 days and continued to ferment for 150 days at room temperature. The results showed that the content of amino nitrogen in fish sauce A and B were 7.6 and 10.6 g/L, respectively. Moreover, the content of total soluble nitrogen in fish sauce A and B were 14.2 and 16.3 g/L, respectively. Only fish sauce B met grade Ⅰ according to standard SB/T 10324—1999. Compared with fish sauce A, the contents of amino nitrogen and total soluble nitrogen in fish sauce B increased by 39.5% and 14.8% after 180 days of fermentation. Besides, the content of free amino acid in fish sauce B, especially glutamic acid, was higher than that in fish sauce A. Meanwhile, GC-MS analysis showed that 3-methyl-butyral and 2-methyl-butyral were only detected in fish sauce B. Furthermore, sensory evaluation showed that fish sauce B had better flavor. In general, all the results demonstrated that the mixed fermentation with exogenous protease and Aspergillus YL001 could shorten the fermentation time and improve the flavor of the fish sauce as well.
陈丽丽, 白春清, 袁美兰, 等.高效液相色谱法测定鱼露中章鱼胺和牛磺酸[J].食品与发酵工业, 2020, 46(2):261-265.CHEN L L, BAI C Q, YUAN M L, et al.Determination of octopamine and taurine in fish sauce by HPLC[J].Food and Fermentation Industries, 2020,46(2):261-265.
[2]
薛佳, 曾名湧.鱼露制品的研究进展[J].肉类研究, 2009(11):89-93.XUE J, ZENG M Y.Progress reaserch of the fish sauce products[J].Meat Reaserch, 2009(11):89-93.
[3]
LOPETCHARAT K, PARK J W.Characteristics of fish sauce made from Pacific whiting and surimi by-products during fermentation stage[J].Journal of Food Science, 2002, 67(2):511-516.
[4]
RABIE M A, NAMIR M, RABIE N A, et al.Acceleration of mackerel fish sauce fermentation via bromelain addition[J].Nutrition and Food Science, 2019, 49(1):47-61.
[5]
TAKANO T, SHOZEN K, SATOMI M, et al.Quality of fish sauce products from recycled by-products from fish gel and kamaboko processing[J].Journal of Food Quality, 2012, 35(3):217-227.
[6]
白政泽, 翁佩芳, 张鑫, 等.秋刀鱼鱼露速酿加工工艺研究[J].食品工业科技, 2017, 38(10):167-172.BAI Z Z, WENG P F, ZHANG X, et al.Study on brew processing of saury fish sauce by accelerating fermentation[J].Science and Technology of Food Industry, 2017, 38(10):167-172.
[7]
ZHONG C, NAKANISHI M, GENG J T, et al.Comparison of non-volatile taste-active components in fish sauce produced from lizardfish Saurida wanieso viscera under different conditions[J].Fisheries Science, 2015, 81(3):581-590.
[8]
CHEN D W, ZHANG M, SHRESTHA S.Compositional characteristics and nutritional quality of Chinese mitten crab (Eriocheir sinensis)[J].Food Chemistry, 2007, 103(4):1 343-1 349.
[9]
GAO P, WANG W, JIANG Q, et al.Effect of autochthonous starter cultures on the volatile flavour compounds of Chinese traditional fermented fish (Suan yu)[J].International Journal of Food Science and Technology, 2016, 51(7):1 630-1 637.
[10]
XU W, YU G, XUE C, et al.Biochemical changes associated with fast fermentation of squid processing by-products for low salt fish sauce[J].Food Chemistry, 2008, 107(4):1 597-1 604.
[11]
PRAPASUWANNAKUL N, SUWANNAHONG K.Chemical composition and antioxidant activity of Klongkone shrimp paste[C].7th World Conference on Educational Sciences.Athens:Alevriadou A, 2015:1 095-1 100.
[12]
GASSEM M A.Microbiological and chemical quality of a traditional salted-fermented fish (Hout-Kasef) product of Jazan Region, Saudi Arabia[J].Saudi Journal of Biological Sciences, 2019, 26(1):137-140.
[13]
ZHAO J, JIANG Q, XU Y, et al.Effect of mixed kojis on physiochemical and sensory properties of rapid-fermented fish sauce made with freshwater fish by-products[J].International Journal of Food Science and Technology, 2017, 52(9):2 088-2 096.
[14]
TUNGKAWACHARA S, PARK J W, CHOI Y J.Biochemical properties and consumer acceptance of pacific whiting fish sauce[J].Journal of Food Science, 2003, 68(3):855-860.
[15]
LOPETCHARAT K, CHOI Y J, PARK J W, et al.Fish sauce products and manufacturing:A review[J].Food Reviews International, 2001, 17(1):65-88.
[16]
GOULAS A E, KONTOMINAS M G.Effect of modified atmosphere packaging and vacuum packaging on the shelf-life of refrigerated chub mackerel (Scomber japonicus):Biochemical and sensory attributes[J].European Food Research and Technology, 2007, 224(5):545-553.
[17]
ZHENG B, LIU Y, HE X, et al.Quality improvement on half-fin anchovy (Setipinna taty) fish sauce by Psychrobacter sp. SP-1 fermentation[J].Journal of the Science of Food and Agriculture, 2017, 97(13):4 484-4 493.
[18]
GAO R, ZHENG Z, ZHOU J, et al.Effects of mixed starter cultures and exogenous L-Lys on the physiochemical and sensory properties of rapid-fermented fish paste using longsnout catfish by-products[J].Lwt-Food Science and Technology, 2019, 108:21-30.
[19]
MIZUTANI T, KIMIZUKA A, RUDDLE K, et al.Chemical components of fermented fish products[J].Journal of Food Composition & Analysis, 1992, 5(2):152-159.
[20]
YANG F, XIA W S, ZHANG X W, et al.A comparison of endogenous and microbial proteolytic activities during fast fermentation of silver carp inoculated with Lactobacillus plantarum[J].Food Chemistry, 2016, 207:86-92.
[21]
李锐, 张海玲, 李旭艳, 等.三种发酵方法制备远东拟沙丁鱼鱼露中鲜味物质的比较[J].食品发酵与工业, 2019, 45(22):143-149.LI R, ZHANG H L, LI X Y, et al.Determination of octopamine and taurine in fish sauce by HPLC[J].Food and Fermentation Industries, 2019,45(22):143-149.
[22]
ZENG X, XIA W, JIANG Q, et al.Contribution of mixed starter cultures to flavor profile of Suanyu-A traditional Chinese low-salt fermented whole fish[J].Journal of Food Processing and Preservation, 2017, 41(5):e13 131.
[23]
FUKAMI K, ISHIYAMA S, YAGURAMAKI H, et al.Identification of distinctive volatile compounds in fish sauce[J].Journal of Agricultural and Food Chemistry, 2002, 50(19):5 412-5 416.
[24]
JIANG J J, ZENG Q X, ZHU Z W.Analysis of volatile compounds in traditional Chinese fish sauce (Yu Lu)[J].Food and Bioprocess Technology, 2011, 4(2):266-271.
[25]
CHEN Q, KONG B, HAN Q, et al.The role of bacterial fermentation in lipolysis and lipid oxidation in Harbin dry sausages and its flavour development[J].LWT-Food Science and Technology, 2017, 77:389-396.
[26]
GIRARD B, DURANCE T.Headspace volatiles of sockeye and pink salmon as affected by retort process[J].Journal of Food Science, 2000, 65(1):34-39.
[27]
YU X, MAO X, HE S, et al.Biochemical properties of fish sauce prepared using low salt, solid state fermentation with anchovy by-products[J].Food Science and Biotechnology, 2014, 23(5):1 497-1 506.
[28]
SUN J, YU X, FANG B, et al.Effect of fermentation by Aspergillus oryzae on the biochemical and sensory properties of anchovy (Engraulis japonicus) fish sauce[J].International Journal of Food Science and Technology, 2016, 51(1):133-141.
2021, Vol. 47 
