Abstract: In this study, hulless barley flour (HBF), insoluble dietary fiber-phenolic compounds (IDF-PC) and soluble dietary fiber-phenolic compounds (SDF-PC) were used as experimental materials to analyze the synergetic regulation of gut microbiota by dietary fiber and polyphenol, according to the abundance and diversity of microbes by in vitro colonic fermentation and 16S rRNA high-throughput sequencing. Ferulic acid (FA) was taken as the control. IDF-PC and SDF-PC were extracted and isolated by enzymatic hydrolytic methods. The total phenolic content was determined using Folin-Ciocalteu assay. Moreover, short-chain fatty acids were analyzed by high performance liquid chromatography. The results showed that HBF, IDF-PC and SDF-PC were rich in dietary fibers and phenolic compounds. After in vitro colonic fermentation, these three samples could improve the abundance and diversity of gut microbiota and promote the growth of bacterial groups, including Lactobacillus, Akkermansia, Ruminococcaceae-UCG-005, Ruminococcaceae-NK4A214-group, compared with FA. Fermentation of HBF, IDF-PC and SDF-PC resulted in an increase in SCFAs production and the peak contents in acetic acid, which were (0.29±0.21), (0.15±0.05) and (0.14±0.10) mg/mL, respectively. This study suggests that hulless barley dietary fibers and phenolic compounds have better synergistic regulation on gut microbiota, which can provide scientific basis for hulless barley health promotion and disease prevention.
鲁朝凤,黄佳琦,黄勇桦,等. 青稞膳食纤维和多酚对肠道微生物的协同调节作用[J]. 食品与发酵工业, 2021, 47(8): 6-13.
LU Chaofeng,HUANG Jiaqi,HUANG Yonghua,et al. Synergetic regulation of gut microbiota by dietary fiber and phenolic compounds in hulless barley[J]. Food and Fermentation Industries, 2021, 47(8): 6-13.
ZHAO L P, ZHANG F, DING X Y, et al.Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes[J].Science, 2018, 359(6 380):1 151-1 156.
ANA E.QUIRÓS S, RAMÓN P, JESU'S F A, et al.Impact of Fruit Dietary Dibers and Polyphenols on Modulation of the Human Fut Microbiota:Chemistry and Human Health[M].2th ed.Hoboken:John Wiley & Sons Ltd, 2017.
DUDA-CHODAK A, TARKO T, SATORA P, et al.Interaction of dietary compounds, especially polyphenols, with the intestinal microbiota:A review[J].European Journal of Nutrition, 2015, 54(3):325-341.
杨华, 叶发银, 赵国华.膳食多酚与肠道微生物相互作用研究进展[J].食品科学, 2015, 36(3):223-227.YANG H, YE F Y, ZHAO G H.Advances in interaction between gut microflora and dietary polyphenols[J].Food Science, 2015, 36(3):223-227.
SÁYAGO-AYERDI S G,ZAMORA-GASGA V M,VENEMA K.Prebiotic effect of predigested mango peel on gut microbiota assessed in a dynamic in vitro model of the human colon (TIM-2)[J].Food Research International, 2019, 118:89-95.
MARTENS E.Gut microbial degradation of complex carbohydrates in colonic health and disease[J].Environmental and Molecular Mutagenesis, 2015, 56:46-46.
NIV Z, GILI Z, JOTHAM S, et al.Personalized gut mucosal colonization resistance to empiric probiotics is associated with unique host and microbiome features[J].Cell, 2018, 174(6):1 388-1 405.
JOTHAM S, NIV Z, GILI Z, et al.Post-antibiotic gut mucosal microbiome reconstitution is impaired by probiotics and improved by autologous FMT[J].Cell, 2018, 174(6):1 406-1 423.
ABBEELE P V D,DUYSBURGH C,JIANG T A, et al.A combination of xylooligosaccharides and a polyphenol blend affect microbial composition and activity in the distal colon exerting immunomodulating properties on human cells[J].Journal of Functional Foods, 2018, 47:163-171.
PAOLA V, AURORA N, VINCENZO F.Cereal dietary fibre:A natural functional ingredient to deliver phenolic compounds into the gut[J].Trends in Food Science & Technology, 2008, 19(9):451-463.
WANG H Y, HONG T, LI N, et al.Soluble dietary fiber improves energy homeostasis in obese mice by remodeling the gut microbiota[J].Biochem Biophys Res Commun, 2018, 498(1):146-151.
DUEÑAS M,MUÑOZ-GONZÁLEZ I,CUEVA C, et al.A survey of modulation of gut microbiota by dietary polyphenols[J].BioMed Research International, 2015.DOI:10.11055/2015/850920.
GONG L X, CAO W Y, GAO J, et al.Whole tibetan hull-less barley exhibit stronger effect on promoting growth of genus bifidobacterium than refined barley in vitro[J].Journal of Food Science, 2018, 83(4):1 116-1 124.
LEE M H. Official methods of analysis of AOAC International (16th edn): Edited by Patricia A. Cunniff, AOAC International, 1995. \$359.00 (North America)/\$399.00 (elsewhere) (xxvi + 1899 pages) ISBN 0 935 584 54 4[J]. Trends in Food Science & Technology, 1995, 6(11):382.
李永强, 杨士花, 吴国星, 等.青稞膳食纤维-多酚复合物的制备方法:云南, CN104382020A[P].2015-03-04.LI Y Q, YANG S H, WU G X, et al.Preparation of Highland Barley Dietary Fiber-polyphenol Complex:Yunnan, CN104382020A[P].2015-03-04.
LI Q, YANG S H, LI Y Q, et al.Comparative evaluation of soluble and insoluble-bound phenolics and antioxidant activity of two chinese mistletoes[J].Molecules, 2018, 23(2):359.
ANOMA C, FEREIDOON S.Bioaccessibility and antioxidant potential of millet grain phenolics as affected by simulated in vitro digestion and microbial fermentation[J].Journal of Functional Foods, 2012, 4(1):226-237.
邹青飞, 杨士花, 李永强, 等.体外结肠发酵对青稞膳食纤维中酚类化合物的含量及抗氧化活性的影响[J].食品科学, 2020, 41(2):94-100.ZOU Q F, YANG S H, LI Y Q, et al.Effects of in vitro colonic fermentation on the content and antioxidant activity of phenolic compounds in dietary fiber of highland barley[J].Food Science, 2020, 41(2):94-100.
AURA A M,HÄRKÖNEN H,FABRITIUS M, et al.Development of an in vitro enzymic digestion method for removal of starch and protein and assessment of its performance using rye and wheat breads[J].Journal of Cereal Science, 1999, 29(2):139-152.
PARKAR S G,TROWER T M,STEVENSON D E.Fecal microbial metabolism of polyphenols and its effects on human gut microbiota[J].Anaerobe, 2013, 23:12-19.
QUAST C, PRUESSE E, YILMAZ P, et al.The SILVA ribosomal RNA gene database project:Improved data processing and web-based tools[J].Nucleic Acids Res, 2013, 41:590-596.
WANG Q, GARRITY G M, TIEDJE J M, et al.Naive bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Appl Environ Microbiol, 2007, 73(16):5 261-5 267.
SCHLOSS P D, GEVERS D, WESTCOTT S L.Reducing the effects of PCR amplification and sequencing artifacts on 16S RNA-based studies[J].Plos One, 2011, 6(12):e27 310.
HUANG J C, CHEN L,XUE B, et al.Different flavonoids can shape unique gut microbiota profile in vitro[J].Journal of Food Ence, 2016, 81(9):2 273-2 279.
LIU S, YU Q, HUANG H, et al.The effect of bound polyphenols on the fermentation and antioxidant properties of carrot dietary fiber in vivo and in vitro[J].Food & Function, 2020, 11(1):748-758.
BISHEHSARI F,ENGEN P A,PREITE N Z, et al.Dietary fiber treatment corrects the composition of gut microbiota, promotes SCFA production, and suppresses colon carcinogenesis[J].Genes, 2018, 9(2):102.
JIN G W, ASOU Y, ISHIYAMA K, et al.Proanthocyanidin-rich grape seed extract modulates intestinal microbiota in ovariectomized mice[J].Journal of Food Ence, 2018, 83(4):1 149-1 152.
LEE H C, JENNER A M,LOW C S, et al.Effect of tea phenolics and their aromatic fecal bacterial metabolites on intestinal microbiota[J].Research in Microbiology, 2006, 157(9):876-884.
陆佳,方秀才.乳杆菌属治疗肠易激综合征的机制[J].胃肠病学和肝病学杂志, 2017, 26(2):218-221.LU J, FANG X C.Mechanism of Lactobacillus in the treatment of irritable bowel syndrome[J].Chinese Journal of Gastroenterology and Hepatology, 2017, 26(2):218-221.
CHENG Y X, WU T, CHU X Q, et al.Fermented blueberry pomace with antioxidant properties improves fecal microbiota community structure and short chain fatty acids production in an in vitro mode[J].LWT - Food Science and Technology, 2020, 125:109-118.
SUN M M, WU W, LIU Z J, et al.Microbiota metabolite short chain fatty acids, GPCR, and inflammatory bowel diseases[J].Journal of Gastroenterology, 2017, 52(1):1-8.
PETRA L, HARRY J.F.Formation of propionate and butyrate by the human colonic microbiota[J].Environmental Microbiology, 2017, 19(1):29-41.
ZORRAQUN-PEÑA I,SÁNCHEZ-HERNÁNDEZ E, PURAN B, et al.Current and future experimental approaches in the study of grape and wine polyphenols interacting gut microbiota[J].Journal of the Ence of Food and Agriculture, 2020, 100(10):3 789-3 802.