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食品与发酵工业  2021, Vol. 47 Issue (16): 46-51    DOI: 10.13995/j.cnki.11-1802/ts.025983
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高剂量摄入火麻仁肽和火麻仁蛋白对小鼠肾功能的影响
朱艳, 毕园, 王雨辰, 马永庆, 王憬, 薛晨, 谷瑞增, 魏颖*
(中国食品发酵工业研究院有限公司 北京蛋白功能肽工程技术研究中心,北京,100015)
Effect of high dose ingestion of hemp seed peptide and protein on renal function of mice
ZHU Yan, BI Yuan, WANG Yuchen, MA Yongqing, WANG Jing, XUE Chen, GU Ruizeng, WEI Ying*
(China National Research Institute of Food and Fermentation Industries Co. Ltd., Beijing Engineering Research Center of Protein & Functional Peptide Corporation Limited, Beijing 100015, China)
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摘要 该文对摄入高剂量火麻仁蛋白和火麻仁肽对正常小鼠或者免疫力低下小鼠肾功能的影响进行了探究。通过连续3 d 腹腔注射60 mg/kg环磷酰胺建立免疫力低下动物模型,正常小鼠不进行造模,连续30 d按照10 g/kg分别灌胃火麻仁蛋白和火麻仁肽,检测血清中尿酸(uric acid,UA)、肌酐(creatinine,CRE)、血尿素氮(blood urea nitrogen,BUN)、免疫球蛋白G(immunoglobulin G,IgG)、免疫球蛋白M(immunoglobulin M,IgM)的含量,肾脏中超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-PX)活力和丙二醛(malondialdehyde,MDA)含量,进一步对小鼠肾脏进行苏木精-伊红(hematoxylin-eosin,HE)染色,观察肾组织病理学变化。结果显示,火麻仁蛋白和火麻仁肽对正常小鼠血清UA、CRE、BUN、IgG和IgM含量以及肾SOD、GSH-PX以及MDA含量无显著影响,且不会引起肾组织损伤。对于免疫力低下小鼠,火麻仁蛋白和火麻仁肽能够降低血清中UN、CRE和BUN含量,增加血清中IgG和IgM含量,通过提高肾组织中SOD、GSH-PX活力和降低MDA含量增加肾脏的抗氧化能力,肾HE染色结果表明,二者能够缓解环磷酰胺引起的肾脏组织病变,且火麻仁肽效果更强。高剂量摄入火麻仁蛋白或火麻仁肽对正常小鼠和免疫力低下小鼠的肾功能均无影响,并具有一定的肾脏保护功能。
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朱艳
毕园
王雨辰
马永庆
王憬
薛晨
谷瑞增
魏颖
关键词:  火麻仁蛋白  火麻仁肽  肾功能  环磷酰胺    
Abstract: The effect of high doses of hemp seed protein or hemp seed peptide on the renal function of normal or immunocompromised mice was studied in this paper. Low immunity animal model was established by intraperitoneal injection of 60 mg/kg cyclophosphamide for 3 d. The hemp seed protein and peptide were fed 10 g/kg for 30 d. Then the content of uric acid (UA), creatinine (CRE), blood urea nitrogen (BUN), immunoglobulin G (IgG) and immunoglobulin M (IgM) in serum were determined, also, the superoxide dismutase (SOD) and glutathion peroxidase (GSH-Px) activity and the content of malondialdehyde (MDA) in the kidney were measured by relevant kits, and the histopathological changes of the kidney in mice were observed by hematoxylin-eosin (HE) staining. Hemp seed protein and peptide had no significant effect on the contents of UN, CRE, BUN, IgG and IgM in serum, as well as the activity of SOD and GSH-Px and the content of MDA in the kidney in normal mice. And the HE staining results showed that these two did not cause damage to kidney tissue for normal mice. For low immunity in mice, hemp seed protein and peptide could reduce the UA, CRE, BUN content in serum, increased the content of serum IgG and IgM, and by increasing the SOD, GSH-Px activity and reducing MDA content in kidney to increase the kidney antioxidant capacity. Moreover, HE staining results also indicated that hemp seed protein and peptide could ease the degree of renal lesions caused by cyclophosphamide. Compared with hemp seed protein, the effect of hempseed peptide was stronger. Therefore, high dose of hemp seed protein and peptide had no damage to renal function in normal and immunocompromised mice, and had a certain effect of renal protection.
Key words:  hempseed protein    hempseed peptide    renal function    cyclophosphamide
收稿日期:  2020-10-23      修回日期:  2020-11-26                发布日期:  2021-09-10      期的出版日期:  2021-08-25
基金资助: 十三五国家重点研发计划项目(2016YFD0400604)
作者简介:  朱艳硕士与毕园学士为共同第一作者(魏颖教授级高级工程师为通讯作者,E-mail:proudwy@126.com)
引用本文:    
朱艳,毕园,王雨辰,等. 高剂量摄入火麻仁肽和火麻仁蛋白对小鼠肾功能的影响[J]. 食品与发酵工业, 2021, 47(16): 46-51.
ZHU Yan,BI Yuan,WANG Yuchen,et al. Effect of high dose ingestion of hemp seed peptide and protein on renal function of mice[J]. Food and Fermentation Industries, 2021, 47(16): 46-51.
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