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食品与发酵工业  2021, Vol. 47 Issue (5): 180-185    DOI: 10.13995/j.cnki.11-1802/ts.026096
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
白酒中特征手性物质的SLE-GC-MS识别方法
王明, 王逍, 陈嘉杰, 吴奕萱, 柯润辉*
(中国食品发酵工业研究院有限公司, 国家食品质量监督检验中心, 北京, 100015)
SLE-GC-MS recognition method of characteristic chiral substances in Baijiu
WANG Ming, WANG Xiao, , CHEN Jiajie, WU Yixuan, KE Runhui*
(China Food fermentation Industry Research Institute Co., Ltd., National Food quality Supervision and Inspection Center, Beijing 100015, China)
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摘要 运用固相支撑-液液萃取(solid-supported liquid-liquid extraction, SLE)技术和手性环糊精气相色谱柱建立了白酒中乳酸乙酯的2种对映体和2, 3-丁二醇的3种对映体的分析方法, 采用内标标准曲线定量。结果表明, 乳酸乙酯、2, 3-丁二醇在各自质量浓度范围内线性关系良好, 在基质样品中的平均加标回收率在80%以上, 相对标准偏差为1.34%~8.50%。该方法测得D-L-乳酸乙酯和S-R-meso-2, 3-丁二醇的检出限分别是64.32、47.74、62.70、59.08和26.58 μg/L。利用所建立方法对酱香、浓香、清香3种香型的6种市售白酒样品进行分析, 目标手性对映体在所选6个酒样中含量及比例不同, 并首次在白酒中分离出S-2, 3-丁二醇。该方法具有高效灵敏、精密度和准确度高、易操作等特点, 适用于白酒中手性物质的检测, 可为利用手性识别进行白酒质量监控、原产地溯源、品质鉴定提供新思路。
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WANG Ming
WANG Xiao
CHEN Jiajie
WU Yixuan
KE Runhui
关键词:  SLE-GC-MS  手性分离  对映体比例  白酒  品质鉴别    
Abstract: Solid-phase support-liquid-liquid extraction (SLE) technique and chiral cyclodextrin column were used to identify two enantiomers (optical isomers) of ethyl lactate and three enantiomers of 2, 3-butanediol in Baijiu. The internal standard curve was used for quantification. The results showed that the linear relationship of ethyl lactate and 2, 3-butanediol was good in certain ranges, the recovery rate in matrix samples was more than 80%, and the relative standard deviation was 1.34%-8.5%. The limits of detection (LODs) of D-ethyl lactate, L-ethyl lactate and S-2, 3-butanediol, R-2, 3-butanediol, meso-2, 3-butanediol were 64.32, 47.74, 62.70, 59.08 and 26.58μg/L, respectively. This method was used to detect six liquor samples of three flavor types. The results showed that the content and proportion of chiral enantiomers were different in six liquor samples, and S-2, 3-butanediol (right-handed) was isolated from liquor for the first time. Due to its high accuracy and good reproducibility, the method could be used for detection of chiral substances in liquor, and can provide a new idea for liquor quality monitoring, origin tracing and quality identification by chiral recognition.
Key words:  SLE-GC-MS    chiral separation    enantiomeric ratio    Baijiu    quality identification
收稿日期:  2020-11-06      修回日期:  2020-11-22                发布日期:  2021-03-31      期的出版日期:  2021-03-15
基金资助: 国家重点研发计划项目(2019YFC1606503)
作者简介:  硕士研究生(柯润辉教授级高级工程师为通讯作者, E-mail:runhuike@163.com)
引用本文:    
WANG Ming,WANG Xiao,CHEN Jiajie,等. 白酒中特征手性物质的SLE-GC-MS识别方法[J]. 食品与发酵工业, 2021, 47(5): 180-185.
王明,王逍,陈嘉杰,et al. SLE-GC-MS recognition method of characteristic chiral substances in Baijiu[J]. Food and Fermentation Industries, 2021, 47(5): 180-185.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.026096  或          http://sf1970.cnif.cn/CN/Y2021/V47/I5/180
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