Protective effect of Ginkgo biloba peptides on acute alcoholic liver injury in mice
ZHENG Yi1,2*, LI Shiying1, MI Xinyi1, CHEN Lin1, LI Chuang1, LIANG Yifan1
1(School of Food and Biological Engineering, Xuzhou University of Technology, Xuzhou 221018, China) 2(Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou 221018, China)
Abstract: Intake of bioactive peptides has been proposed as an effective adjuvant therapy for acute alcoholic liver injury by ameliorating alcohol-induced oxidative stress and inflammatory damage. This paper investigated the hepatoprotective effect of Ginkgo biloba peptides (GBP) on acute alcoholic liver injury in mice. Male Kunming mice were randomly divided into six groups, including normal group, model group, GBP low-dose (50 mg/kg), GBP medium-dose (100 mg/kg), GBP high-dose group (200 mg/kg) and positive control group (100 mg/kg reduced glutathione). An acute alcoholic liver injury mice mode was established by ethanol exposure. The kits were employed to determine the levels of the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α). And the levels of the liver tissue catalase (CAT), glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), malondialdehyde (MDA) and protein carbonyl group (PCG). The results showed that GBP treatment reduced the levels of ALT, AST, TG and TC, down-regulated the levels of pro-inflammatory cytokines including IL-1β, IL-6 and TNF-α. Besides, GBP treatment also increased the CAT, GSH-Px and T-SOD activities and decreased MDA and PCG levels. The results indicated that GBP exerted the hepatoprotective effect on acute alcoholic liver injury in mice through antioxidant and anti-inflammatory pathways.
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