Please wait a minute...
 
 
食品与发酵工业  2021, Vol. 47 Issue (20): 247-252    DOI: 10.13995/j.cnki.11-1802/ts.027093
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
微波等离子体原子发射光谱分析富硒农产品中总硒
江波1, 陈林2*
1(长江师范学院 现代农业与生物工程学院,重庆 涪陵,408100)
2(湖南中医药研究院 中医研究所,湖南 长沙,410013)
Total selenium analysis in selenium-enriched agricultural products with microwave plasma atomic emission spectrometry
JIANG Bo1, CHEN Lin2*
1(School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Fuling 408100, China)
2(Institute of Chinese Materia Medica, Hunan Academy of Traditional Chinese Medicine, Changsha 410013, China)
下载:  HTML  PDF (1929KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 建立微波等离子体原子发射光谱(microwave plasma atomic emission spectrometry,MP-AES)法测定富Se农产品中总Se含量的分析方法。采用微波消解对样品进行预处理,采用盐酸将Se(Ⅵ)还原为Se(Ⅳ),在多模式样品导入系统中使用L-半胱氨酸/酒石酸与消解溶液在线混合提高Se(Ⅳ)的还原效率,利用NaBH4/NaOH将Se(Ⅳ)还原为H2Se,采用MP-AES进行测定。选择196.026 nm为Se的分析波长,可避开光谱重叠干扰,利用快速线性干扰校正技术校正背景干扰。采用国家标准参考物质验证方法的准确性,总Se的测定结果与标准参考物质的参考值基本一致。Se的检出限(limit of detection,LOD)为0.58 μg/L,不同富Se农产品中总Se的含量为0.11~1.84 μg/g。MP-AES利用配备的N2发生器产生大量N2取代气瓶或液体储存系统降低分析成本,提高分析效率,并且灵敏度和LOD俱佳。该方法可用于富Se农产品中总Se含量的准确测定。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
江波
陈林
关键词:  富硒农产品  总硒  微波等离子体原子发射光谱  多模式样品导入系统  快速线性干扰校正    
Abstract: Analytical method was established for the determination of total selenium (Se) concentrations in selenium-enriched agricultural products using microwave plasma atomic emission spectrometry (MP-AES). After microwave digestion of selenium-enriched agricultural products, Se (Ⅵ) was reduced to Se (Ⅳ) by HCl. In the multimode sample introduction system (MSIS), the reduction efficiency of Se (Ⅵ) was improved by on-line mixing of L-cysteine/tartaric acid with the sample solution, Se (Ⅳ) was reduced to H2Se using NaBH4/NaOH, and MP-AES was used for determination. And 196.026 nm was selected as the analysis wavelength of Se to avoid spectral interference. The fast-linear interference correction (FLIC) model was used to correct the background interference. The national standard reference materials were used to verify the accuracy of the method, and the determination results of total Se were consistent with the certified value of the standard reference materials. Furthermore, the limit of detection (LOD) was 0.58 μg/L, and the total Se content in different selenium-enriched agricultural products was between 0.11-1.84 μg/g. In addition, MP-AES reduced the analytical cost and improved the analytical efficiency by replacing bottles or liquid storage systems with abundantly available nitrogen from a generator. With good sensitivity and LOD, this method can be used for the accurate determination of total Se concentrations in selenium-enriched agricultural products.
Key words:  selenium-enriched agricultural products    total selenium    microwave plasma atomic emission spectrometry    multimode sample introduction system    fast linear interference correction
收稿日期:  2021-02-20      修回日期:  2021-03-02           出版日期:  2021-10-25      发布日期:  2021-11-18      期的出版日期:  2021-10-25
基金资助: 国家自然科学基金项目(81603400);重庆市自然科学基金项目(cstc2018jcyjAx0594)
作者简介:  硕士,副教授(陈林助理研究员为通讯作者,E-mail:lchencs@163.com)
引用本文:    
江波,陈林. 微波等离子体原子发射光谱分析富硒农产品中总硒[J]. 食品与发酵工业, 2021, 47(20): 247-252.
JIANG Bo,CHEN Lin. Total selenium analysis in selenium-enriched agricultural products with microwave plasma atomic emission spectrometry[J]. Food and Fermentation Industries, 2021, 47(20): 247-252.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.027093  或          http://sf1970.cnif.cn/CN/Y2021/V47/I20/247
[1] CARDOSO B R, HARE D J, BUSH A I, et al.Selenium levels in serum, red blood cells, and cerebrospinal fluid of Alzheimer′s disease patients:A report from the Australian imaging, biomarker & lifestyle flagship study of ageing (AIBL)[J].Journal of Alzheimer’s Disease, 2017, 57:183-193.
[2] RAYMAN M P.Selenium and human health[J].Lancet, 2012, 379:1 256-1 268.
[3] Food and Nutrition Board, Institute of Medicine.Dietary Reference Intakes from Vitamin C, Vitamin E, Selenium and Carcinoids[M].New York:National Academic Press, 2000.
[4] LIU L, YUN Z, HE B, et al.Efficient interface for online coupling of capillary electrophoresis with inductively coupled plasma-mass spectrometry and its application in simultaneous speciation analysis of arsenic and selenium[J].Analytical Chemistry, 2014, 86(16):8 167-8 175.
[5] MICKE O, SCHOMBURG L, BUENTZEL J, et al.Selenium in oncology:From chemistry to clinics[J].Molecules, 2009, 14:3 975-3 988.
[6] 施怡, 胡兴锁, 潘亚璐, 等.富硒双孢蘑菇硒形态分布及其营养品质和抗氧化特性[J].食品科学, 2017, 38(9):99-105.
SHI Y, HU X S, PAN Y L, et al.Selenium speciation distribution, nutritional quality and antioxidant activity of Se-enriched Agaricus bisporus[J].Food Science, 2017, 38(9):99-105.
[7] DA SILVA D L F, DA COSTA M A P, SILVA L O B, et al.Simultaneous determination of mercury and selenium in fish by CVG AFS[J].Food Chemistry, 2019, 273:24-30.
[8] MAZEJ D, FALNOGA I, VEBER M, et al.Determination of selenium species in plant leaves by HPLC-UV-HG-AFS[J].Talanta, 2006, 68(3):558-568.
[9] SHALTOUT A A, BOUSLIMI J, BESBES H.The challenges of Se quantification in bean samples using line and continuum sources atomic absorption spectrometry[J].Food Chemistry, 2020, 328:127 124.
[10] 邓世林, 李新凤, 郭小林.流动注射氢化物发生原子吸收光谱法测定禽蛋中的硒[J].光谱学与光谱分析, 2010, 30(3):809-811.
DENG S L, LI X F, GUO X L.Determination of selenium in poultry eggs by flow injection-hydride generation-atomic absorption spectrometry[J].Spectroscopy and Spectral Analysis, 2010, 30(3):809-811.
[11] GHASEMI E, NAJAFI N M, RAOFIE F, et al.Simultaneous speciation and preconcentration of ultra traces of inorganic tellurium and selenium in environmental samples by hollow fiber liquid phase microextraction prior to electrothermal atomic absorption spectroscopy determination[J].Journal of Hazardous Materials, 2010, 181(1-3):491-496.
[12] ESCUDERO L A, PACHECO P H, GASQQUEZ J A, et al.Development of a FI-HG-ICP-OES solid phase preconcentration system for inorganic selenium speciation in Argentinean beverages[J].Food Chemistry, 2015, 169:73-79.
[13] 黄小兰, 何旭峰, 周祥德, 等.不同产地地参中22种元素的测定与分析[J].食品与发酵工业, 2020, 46(20):229-235.
HUANG X L, HE X F, ZHOU X D, et al.Determination and analysis of 22 elements in Lycopus lucidus var.hirtus Regel from different habitats[J].Food and Fermentation Industries, 2020, 46(20):229-235.
[14] ALTUNAY N, GURKAN L R.Determination of sub-ng g–1 levels of total inorganic arsenic and selenium in foods by hydride-generation atomic absorption spectrometry after pre-concentration[J].Food Additives & Contaminants:Part A, 2017, 34(3):390-403.
[15] BAMONTI L, THEINER S, ROHR-UDILOVA N, et al.Accurate high throughput quantification of selenium in biological samples-the potential of combining isotope dilution ICP-tandem mass spectrometry with flow injection[J].Journal of Analytical Atomic Spectrometry, 2016, 31(11):2 227-2 232.
[16] HU X, CAO Z, SUN W, et al.Accurate determination of arsenic and selenium in plant food samples by using ICP-MS/MS[J].Analytical Methods, 2016, 8(32):6 150-6 157.
[17] TANABE C K, HOPFER H, GILLELANG G, et al.Total arsenic analysis in Californian wines with hydride generation-microwave plasma-atomic emission spectroscopy (HG-MP-AES)[J].Journal of Analytical Atomic Spectrometry, 2016, 31(6):1 223-1 227.
[18] ZHAO Y, LI Z, ROSS A, et al.Determination of heavy metals in leather and fur by microwave plasma-atomic emission spectrometry[J].Spectrochimica Acta Part B:Atomic Spectroscopy, 2015, 112:6-9.
[19] 张萍, 刘宏伟.微波等离子体原子发射光谱法测定食用坚果油中的金属元素[J].食品与发酵工业, 2020, 46(18):215-218.
ZHANG P, LIU H W.Determination of metal elements in edible nut oil by microwave plasma atomic emission spectroscopy[J].Food and Fermentation Industries, 2020, 46(18):215-218.
[20] 戴亚明. 硒、碲氢化物发生的反应机理和反应条件[J].理化检验-化学分册, 2007, 43(11):901-905.
DAI Y M.Mechanism and conditions for the hydride formation reactions of selenium and tellurium[J].Physical Testing and Chemical Analysis Part B:Chemical Analysis, 2007, 43(11):901-905.
[21] 杨熙, 潘佳钏, 雷永乾, 等.微波等离子体发射光谱法同时测定特色南药中多种元素[J].分析测试学报, 2015, 34(2):227-231.
YANG X, PAN J C, LEI Y Q, et al.Simultaneous determination of multiple elements in characteristic south China herbal medicine by microwave plasma atomic emission spectrometry[J].Journal of Instrumental Analysis, 2015, 34(2):227-231.
[22] 中华人民共和国国家卫生和计划生育委员会. GB 5009.93—2017 食品安全国家标准 食品中硒的测定[S].北京:中国标准出版社, 2017.
National Health and Family Planning Commission of the People′s Republic of China.GB 5009.93—2017 National food safety standard Determination of selenium in foods[S].Beijing:Standards Press of China, 2017.
[23] 邹春苗, 刘静晶, 廖雪, 等.电感耦合等离子体质谱法和氢化物原子荧光光谱法测定富硒大米中硒含量的对比分析[J].食品安全质量检测学报, 2020, 11(4):1 231-1 234.
ZOU C M, LIU J J, LIAO X, et al.Comparative analysis of determination of selenium in selenium-enriched rice by inductively coupled plasma mass spectrometry and hydride generation-atomic fluorescence spectrometry[J].Journal of Food Safety & Quality, 2020, 11(4):1 231-1 234.
[24] 赵萍, 刘笑笑, 王雅, 等.富硒小麦提取物中硒含量及其抗氧化特性[J].食品科学, 2014, 35(15):94-98.
ZHAO P, LIU X X, WANG Y, et al.Selenium content and antioxidant activity of selenium-enriched wheat[J].Food Science, 2014, 35(15):94-98.
[25] 梁克红, 卢林纲, 朱大洲, 等.我国小米硒含量情况及富硒化研究进展[J].中国食品学报, 2020, 20(7):337-343.
LIANG K H, LU L G, ZHU D Z, et al.Research progress of selenium content and selenium enrichment in millet of China[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(7):337-343.
[26] 孙向东, 兰静, 张瑞英, 等.我国普通和富硒黑木耳及香菇硒含量分析[J].农产品质量与安全, 2021(1):38-41.
SUN X D, LAN J, ZHANG R Y, et al.Analysis of selemium content in common and selenium enriched Auricularia auricular and Lentinus edodes in China[J].Quality and Safety of Agro-Products, 2021(1):38-41.
[27] 林毅韵, 李琼, 朱家俊, 等.超级微波-电感耦合等离子体质谱法鉴定富硒茶叶真伪[J].食品安全质量检测学报, 2019, 10(13):4 256-4 260.
LIN Y Y, LI Q, ZHU J J, et al.Identification of rich-selenium tea authenticity by ultra-microwave digestion-inductively coupled plasma mass spectrometry[J].Journal of Food Safety & Quality, 2019, 10(13):4 256-4 260.
[28] 唐荣, 李亚妮, 王鸿赋, 等.三个不同省份富硒大蒜硒含量分析[J].中国食物与营养, 2020, 26(9):26-31.
TANG R, LI Y N, WANG H F, et al.Analysis on selenium content of selenium-enriched garlic in three different provinces[J].Food and Nutrition in China, 2020, 26(9):26-31.
[29] 杨玉玲, 刘元英.富硒大豆中硒的分布研究[J].大豆科学, 2014, 33(4):610-612.
YANG Y L, LIU Y Y.Study on the distribution of selenium in selenite-enrich soybean[J].Soybean Science, 2014, 33(4):610-612.
[30] 张驰, 刘信平, 周大寨, 等.营养元素硒在富硒玉米中的赋存形态及分布研究[J].食品研究与开发, 2007, 28(9):25-27.
ZHANG C, LIU X P, ZHOU D Z, et al.Study on the distribution and combined forms of microelement selenium in corn[J].Food Research and Development, 2007, 28(9):25-27.
[1] 张萍, 刘宏伟. 微波等离子体原子发射光谱法测定食用坚果油中的金属元素[J]. 食品与发酵工业, 2020, 46(18): 215-218.
No Suggested Reading articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
版权所有 © 《食品与发酵工业》编辑部
地址:北京朝阳区酒仙桥中路24号院6号楼111室
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn