The detection of biogenic amines in skipjack tuna by using the ultrahigh performance liquid chromatography-tandem mass spectrometry method
LIU Yangfan1,2, LI Xupeng3, FENG Yang2,4, WANG Di2,4, CHEN Shengjun2,4, YANG Xianqing2,4, WU Yanyan2,4, DENG Jianchao2,4*
1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China) 2(Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China) 3(Guangdong Agricultural Technology Extension Center, Guangzhou 510300, China) 4(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China)
Abstract: This study established a detection method for spermine, spermidine, tyramine, tryptamine, histamine, β-phenylethylamine, putrescine and cadaverine for 8 kinds of biogenic amines by utilizing ultrahigh performance liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS). The sample was extracted and decontaminated by 0.2% acidified acetonitrile and dichloromethane separately, and then separated using a C18 column. Methanol and 0.5% formic acid in water were used as the mobile phase for gradient elution. Mass spectrometry data were obtained by electrospray positive ion (ESI+) scanning in multiple reaction monitoring (MRM) mode, and an internal standard method was used for quantification. Results showed that 8 kinds of biogenic amines could be separated within 6 minutes, and the linearity was good with the correlation coefficient R between 0.995 6 and 0.999 6. The detection limit was in the range of 75-150 μg/kg and the quantification limit was in the range of 250-500 μg/kg. Moreover, the average recovery rate of the sample was between 82%-113% and the relative standard deviation was between 2.3% and 9.7%. This method developed here was simple, fast, and had good sensitivity, recovery, and precision, which was suitable for the determination of biogenic amines in skipjack tuna.
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2022, Vol. 48 
