Abstract: Potato porous starch (PS) is used as a material to prepare carboxymethyl porous starch (CMPS) with better solubility and adsorption, then CMPS will be used as a carrier of biologically active substances. The experiment used N2-adsorption/desorption, scanning electron microscopy (SEM), liquid nuclear magnetic (1H NMR), adsorption performance analysis and other methods to study the characteristics of the prepared samples CMPS. The results showed that carboxymethyl porous starch (CMPS) with a degree of substitution (DS) of 0.25-1.44 was successfully prepared. The specific surface area of PS (42.88 m2/g) was the largest. After carboxymethylation, the specific surface area (12.06 m2/g) of CMPS decreased. However, compared with the specific surface area (0.87 m2/g) of carboxymethyl starch (CMS), it was still significantly increased. Both porous and carboxymethyl treatments made the starch crystal disappear, and the etherification reaction reduced the thermal stability of the starch. The loading rate of CMPS@Q was 13.71%, which was 95.84% higher than the loading rate of CMS@Q (0.57%). Compared with quercetin insoluble in water, the water solubility of CMPS@Q (34.00%) was 45.74% higher than that of CMS@Q (23.33%). The prepared CMPS has a good porous structure, specific surface area, solubility and adsorption performance, and can be used as a carrier of low water-soluble functional substances.
王宇霞,马云翔,苟丽娜,等. 羧甲基多孔淀粉表征及其对槲皮素吸附研究[J]. 食品与发酵工业, 2022, 48(20): 105-111.
王宇霞,马云翔,苟丽娜,et al. The characterization of carboxymethyl porous starch and its adsorption on quercetin[J]. Food and Fermentation Industries, 2022, 48(20): 105-111.
LIU J, WANG X C, YONG H M, et al.Preparation, characterization, digestibility and antioxidant activity of quercetin grafted Cynanchum auriculatum starch[J].International Journal of Biological Macromolecules, 2018, 114:130-136.
[4]
卢双彦, 张雅楠, 翟光喜.槲皮素纳米制剂研究进展[J].药物生物技术, 2019, 26(6):552-557.LU S Y, ZHANG Y N, ZHAI G X.Development of the quercetin loaded nanoformulations[J].Pharmaceutical Biotechnology, 2019, 26(6):552-557.
[5]
LIN J, TEO L M, LEONG L P, et al.In vitro bioaccessibility and bioavailability of quercetin from the quercetin-fortified bread products with reduced glycemic potential[J].Food Chemistry, 2019, 286:629-635.
[6]
CHEN J H, WANG Y X, LIU J, et al.Preparation, characterization, physicochemical property and potential application of porous starch:A review[J].International Journal of Biological Macromolecules, 2020, 148:1 169-1 181.
[7]
JU J, CHEN X Q, XIE Y F, et al.Simple microencapsulation of plant essential oil in porous starch granules:Adsorption kinetics and antibacterial activity evaluation[J].Journal of Food Processing and Preservation, 2019, 43(10):e14156.
[8]
WANG H L, LYU J, JIANG S W, et al.Preparation and characterization of porous corn starch and its adsorption toward grape seed proanthocyanidins[J].Starch-Stárke, 2016, 68(11-12):1 254-1 263.
[9]
HU J, TIAN T, XIAO Z B.Preparation of cross-linked porous starch and its adsorption for chromium (VI) in tannery wastewater[J].Polymers for Advanced Technologies, 2015, 26(10):1 259-1 266.
[10]
BUDARIN V, CLARK J H, HARDY J J E, et al.Starbons:New starch-derived mesoporous carbonaceous materials with tunable properties[J].Angewandte Chemie International Edition, 2006, 45(23):3 782-3 786.
[11]
ZHANG B, LI X M, XIE Q T, et al.Preparation and characterization of non-crystalline granular starch and corresponding carboxymethyl starch[J].International Journal of Biological Macromolecules, 2017, 103:656-662.
[12]
陈庆. 直链淀粉的羧化和多孔改性及其对氨和苯酚的吸附性能研究[D].杭州:浙江大学, 2017.CHEN Q.Study on carboxylation, pore-forming of amylose and its ammonia and phenol adsorption properties[D].Hangzhou:Zhejiang University, 2017.
[13]
AHMAD M, MUDGIL P, GANI A, et al.Nano-encapsulation of catechin in starch nanoparticles:Characterization, release behavior and bioactivity retention during simulated in-vitro digestion[J].Food Chemistry, 2019, 270:95-104.
[14]
刘维信, 冯希环, 蔡宋宋, 等.大葱槲皮素含量的测定[J].中国农学通报, 2008,24(3):266-269.LIU W X, FENG X H, CAI S S, et al.Evaluation of quercetin content in Welsh onion[J].Chinese Agricultural Science Bulletin, 2008,24(3):266-269.
[15]
毕英慧. 羧甲基马铃薯淀粉的制备、反应动力学及性能研究[D].兰州:兰州大学, 2009.BI Y H.Study on the preparation, reaction kinetics and properties of carboxymethyl potato starch[D].Lanzhou:Lanzhou University, 2009.
[16]
李海燕, 马云翔, 俞力月,等.辛烯基琥珀酸酐改性多孔淀粉结构表征[J].中国粮油学报, 2020, 35(6):70-75.LI H Y, MA Y X, YU L Y, et al.Structure characterizatin of octenyl succinic anhydride modified porous starch[J].Journal of the Chinese Cereals and Oils Association, 2020, 35(6):70-75.
[17]
WANG L F, PAN S Y, HU H, et al.Synthesis and properties of carboxymethyl kudzu root starch [J].Carbohydrate Polymers, 2010, 80(1):174-179.
[18]
ZHANG B, CUI D P, LIU M Z, et al.Corn porous starch:Preparation, characterization and adsorption property[J].International Journal of Biological Macromolecules, 2012, 50(1):250-256.
[19]
苟丽娜, 马云翔, 王宇霞, 等.高比表面积阿魏酸多孔淀粉酯结构表征及体外消化特性[J].食品与发酵工业, 2021,47(22):76-83.GOU L N, MA Y X, WANG Y X, et al.Structural characterization and in vitro digestibility of ferulic acid porous starch ester with high specific surface area[J].Food and Fermentation Industries, 2021,47(22):76-83.
[20]
LIU Y, LU K Y, HU X T, et al.Structure, properties and potential applications of phytoglycogen and waxy starch subjected to carboxymethylation[J].Carbohydrate Polymers, 2020, 234:115908.
[21]
NAMAZI H, FATHI F, DADKHAH A.Hydrophobically modified starch using long-chain fatty acids for preparation of nanosized starch particles[J].Scientia Iranica, 2011, 18(3):439-445.
[22]
ZHOU M, SHI L, CHENG F, et al.High-efficient preparation of carboxymethyl starch via ball milling with limited solvent content[J].Starch - Stárke, 2018, 70(5-6):1700250.
[23]
HE X, GONG X C, LI W F, et al.Preparation and characterization of amphiphilic composites made with double-modified (etherified and esterified) potato starches[J].Starch-Stárke, 2019, 71(9-10):1900089.
[24]
SING, K S W.Reporting physisorption data for gas/solid systems with special reference to the determination of surface area and porosity (Recommendations 1984)[J].Pure and Applied Chemistry, 1985, 57(4), 603-619.
[25]
UBEYITOGULLARI A, CIFTCI O N.Generating phytosterol nanoparticles in nanoporous bioaerogels via supercritical carbon dioxide impregnation:Effect of impregnation conditions[J].Journal of Food Engineering, 2017, 207:99-107.
[26]
LI X, GAO W Y, HUANG L J, et al.Preparation and physicochemical properties of carboxymethyl Fritillaria ussuriensis Maxim.starches[J].Carbohydrate Polymers, 2010, 80(3):768-773.
[27]
SUJKA M, PANKIEWICZ U, KOWALSKI R, et al.Porous starch and its application in drug delivery systems[J].Polymers in Medicine, 2018, 48(1):25-29.