Study on the correlation between free amino acid content and taste of rainbow trout (Oncorhynchus mykiss) with different sizes based on UPLC-Q-Trap-MS combined with chemometrics
BAO Shoumin1, CHEN Shengrong1, TIAN Haining2, MENG Yuqiong2*, MA Rui1*
1(State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China) 2(College of Eco-Environmental Engineering, Qinghai University, Xining 810016, China)
Abstract: A method for the analysis of free amino acids in fillets by ultra-performance liquid chromatography coupled with triple quadrupole linear ion trap mass spectrometer(UPLC-Q-Trap-MS) was established. Combined with chemometrics, the correlation between the content of 16 free amino acids and the taste of fillets from rainbow trout (Oncorhynchus mykiss) of different sizes was studied. The analysis conditions were as follows: The chromatographic separation was performed using an Acquity UPLC BEH Amide (2.1 mm×100 mm, 1.7 μm) column and the temperature of the column was maintained at 35 ℃. The mobile phases were 5 mmol/L ammonium formate in water containing 0.10% formic acid and 5 mmol/L ammonium formate in acetonitrile containing 0.10% formic acid, respectively, using a binary gradient elution at the flow rate of 0.3 mL/min. The detection was carried out by electrospray ionization in positive modes and multiple reaction monitoring modes. The difference in taste profiles was investigated by taste activity value and principal component analysis. Results showed that the calibration curves of 16 kinds of amino acids exhibited good linearities within their test ranges (r>0.99). The average recovery rates of the free amino acids in the rainbow trout sample ranged from 98.38%-103.57% and RSD was 0.34%-2.89% (n=6). The contents of free amino acids and the total taste activity value were significantly different in fillets of rainbow trout with different body sizes (P<0.01). The larva rainbow trout tasted sweet and bitter characteristics and the middle rainbow trout showed fresh, sweet and bitter taste characteristics. While the mature rainbow trout showed only bitter taste characteristics. This study provides a reference for the evaluation of the fillet quality of rainbow trout.
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