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食品与发酵工业  2022, Vol. 48 Issue (20): 293-299    DOI: 10.13995/j.cnki.11-1802/ts.030316
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袁婷婷1, 周子艺1, 赵吉春1, 李富华1, 曾凯芳1,2, 明建1,2*
1(西南大学 食品科学学院,重庆,400715)
The interactions between proteins and anthocyanins based on covalent/ non-covalent binding: A review
YUAN Tingting1, ZHOU Ziyi1, ZHAO Jichun1, LI Fuhua1, ZENG Kaifang1,2, MING Jian1,2*
1(College of Food Science, Southwest University, Chongqing 400715, China)
2(Research Center of Food Storage and Logistics, Southwest University, Chongqing 400715, China)
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摘要 蛋白质是生命活动的物质基础,是食品营养的重要成分。花色苷是广泛存在于自然界的植物色素,具有抗氧化、抗炎、抗衰老、抗癌等生理活性。花色苷对环境十分敏感,容易发生降解或分解,但通过与蛋白质的共价和非共价相互作用可以改善这一现象,同时也可以改善蛋白质的功能特性和营养品质。该文综述了花色苷与蛋白质的相互作用形式和影响因素,分别分析了二者的相互作用对花色苷和蛋白质性质的影响,为开发富含花青素的蛋白质食品或功能性配料提供理论依据。
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关键词:  花色苷  蛋白质  相互作用    
Abstract: Protein is the material basis of life activities and an important component of food nutrition. Anthocyanins are plant pigments that are widely found in nature. They have anti-oxidation, anti-inflammatory, anti-ageing, anti-cancer, and other physiological activities. Anthocyanins are very sensitive to the environment and are prone to degradation or decomposition. However, covalent and non-covalent interactions with proteins can improve this phenomenon, as well as the functional properties and nutritional quality of proteins. This article reviewed the interaction forms and influencing factors of anthocyanins and proteins and analyzed the effects of their interactions on the properties of anthocyanins and proteins, providing a theoretical basis for the development of anthocyanin-rich protein foods or functional ingredients.
Key words:  anthocyanin    protein    interaction
收稿日期:  2021-12-03      修回日期:  2021-01-05           出版日期:  2022-10-25      发布日期:  2022-11-18      期的出版日期:  2022-10-25
基金资助: 国家重点研发计划专项课题(2019YFD1002300)
作者简介:  硕士研究生(明建教授为通信作者,
袁婷婷,周子艺,赵吉春,等. 基于共价/非共价结合的蛋白质与花色苷相互作用研究进展[J]. 食品与发酵工业, 2022, 48(20): 293-299.
袁婷婷,周子艺,赵吉春,et al. The interactions between proteins and anthocyanins based on covalent/ non-covalent binding: A review[J]. Food and Fermentation Industries, 2022, 48(20): 293-299.
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[1] LI X, XU J M, TANG X, et al.Anthocyanins inhibit trastuzumab-resistant breast cancer in vitro and in vivo[J].Molecular Medicine Reports, 2016, 13(5):4 007-4 013.
[2] 刘建国, 司旭, 田金龙, 等.花色苷的营养吸收及稳定性研究进展[J].中国果菜, 2020, 40(5):7-13.LIU J G, SI X, TIAN J L, et al.Advances in nutritional absorption and stabilization of anthocyanins[J].China Fruit & Vegetable, 2020, 40(5):7-13.
[3] ERTAN K, TÜRKYιLMAZ M, ÖZKAN M.Effects of natural copigment sources in combination with sweeteners on the stability of anthocyanins in sour cherry nectars[J].Food Chemistry, 2019, 294:423-432.
[4] XIE L H, SU H M, SUN C D, et al.Recent advances in understanding the anti-obesity activity of anthocyanins and their biosynthesis in microorganisms[J].Trends in Food Science & Technology, 2018, 72:13-24.
[5] BUENO J M, SÁEZ-PLAZA P, RAMOS-ESCUDERO F, et al.Analysis and antioxidant capacity of anthocyanin pigments.part II:Chemical structure, color, and intake of anthocyanins[J].Critical Reviews in Analytical Chemistry, 2012, 42(2):126-151.
[6] SUI X N, ZHANG T Y, JIANG L Z.Soy protein:Molecular structure revisited and recent advances in processing technologies[J].Annual Review of Food Science and Technology, 2021, 12:119-147.
[7] LANG Y X, GAO H Y, TIAN J L, et al.Protective effects of α-casein or β-casein on the stability and antioxidant capacity of blueberry anthocyanins and their interaction mechanism[J].LWT, 2019, 115:108434.
[8] HE W J, MU H B, LIU Z M, et al.Effect of preheat treatment of milk proteins on their interactions with cyanidin-3-O-glucoside[J].Food Research International, 2018, 107:394-405.
[9] ZANG Z H, CHOU S R, TIAN J L, et al.Effect of whey protein isolate on the stability and antioxidant capacity of blueberry anthocyanins:A mechanistic and in vitro simulation study[J].Food Chemistry, 2021, 336:127700.
[10] NAS J S.Exploring the binding affinity and non-covalent interactions of anthocyanins with aging-related enzymes through molecular docking[J].Journal of Health Research, 2020, 24:9-19.
[11] QUAN T H, BENJAKUL S, SAE-LEAW T, et al.Protein-polyphenol conjugates:Antioxidant property, functionalities and their applications[J].Trends in Food Science & Technology, 2019, 91:507-517.
[12] TANTOUSH Z, STANIC D, STOJADINOVIC M, et al.Digestibility and allergenicity of β-lactoglobulin following laccase-mediated cross-linking in the presence of sour cherry phenolics[J].Food Chemistry, 2011, 125(1):84-91.
[13] SUI X N, SUN H B, QI B K, et al.Functional and conformational changes to soy proteins accompanying anthocyanins:Focus on covalent and non-covalent interactions[J].Food Chemistry, 2018, 245:871-878.
[14] CAHYANA Y, GORDON M H.Interaction of anthocyanins with human serum albumin:Influence of pH and chemical structure on binding[J].Food Chemistry, 2013, 141(3):2 278-2 285.
[15] MIYAGUSUKU-CRUZADO G, JIMÉNEZ-FLORES R, GIUSTI M M.Whey protein addition and its increased light absorption and tinctorial strength of model solutions colored with anthocyanins[J].Journal of Dairy Science, 2021, 104(6):6 449-6 462.
[16] 张娇娇. 食用花色苷与常见内源性蛋白质相互作用研究[D].杭州:浙江大学, 2019.ZHANG J J.Study on interactions between dietary anthocyanins and common endogenous proteins[D].Hangzhou:Zhejiang University, 2019.
[17] BOHIN M C, VINCKEN J P, VAN DER HIJDEN H T W M, et al.Efficacy of food proteins as carriers for flavonoids[J].Journal of Agricultural and Food Chemistry, 2012, 60(16):4 136-4 143.
[18] 赵焕焦. 黑米花色苷与三种蛋白质相互作用的研究[D].天津:天津科技大学, 2017.ZHAO H J.Study on the interaction of black rice anthocyanins with three proteins[D].Tianjin:Tianjin University of Science & Technology, 2017.
[19] CHEN Z Q, WANG C, GAO X D, et al.Interaction characterization of preheated soy protein isolate with cyanidin-3-O-glucoside and their effects on the stability of black soybean seed coat anthocyanins extracts[J].Food Chemistry, 2019, 271:266-273.
[20] ARROYO-MAYA I J, CAMPOS-TERÁN J, HERNÁNDEZ-ARANA A, et al.Characterization of flavonoid-protein interactions using fluorescence spectroscopy:Binding of pelargonidin to dairy proteins[J].Food Chemistry, 2016, 213:431-439.
[21] FU X Z, BELWAL T, HE Y H, et al.Interaction and binding mechanism of cyanidin-3-O-glucoside to ovalbumin in varying pH conditions:A spectroscopic and molecular docking study[J].Food Chemistry, 2020, 320:126616.
[22] ZHOU R, DONG X Y, SONG L L, et al.Interaction mode and nanoparticle formation of bovine serum albumin and anthocyanin in three buffer solutions[J].Journal of Luminescence, 2014, 155:244-250.
[23] TORRES A, AGUILAR-OSORIO G, CAMACHO M, et al.Characterization of polyphenol oxidase from purple sweet potato (Ipomoea batatas L.Lam) and its affinity towards acylated anthocyanins and caffeoylquinic acid derivatives[J].Food Chemistry, 2021, 356:129709.
[24] STÜBLER A S, LESMES U, JUADJUR A, et al.Impact of pilot-scale processing (thermal, PEF, HPP) on the stability and bioaccessibility of polyphenols and proteins in mixed protein- and polyphenol-rich juice systems[J].Innovative Food Science & Emerging Technologies, 2020, 64:102426.
[25] 周瑞, 董学艳, 景浩.不同溶液中牛血清白蛋白与花青素相互作用特征及抗氧化性[J].食品科学, 2013, 34(15):11-16.ZHOU R, DONG X Y, JING H.Characterization of bovine serum albumin/anthocyanin interaction and antioxidant activity in different solutions[J].Food Science, 2013, 34(15):11-16.
[26] QIN X G, YUAN D, WANG Q, et al.Maillard-reacted whey protein isolates enhance thermal stability of anthocyanins over a wide pH range[J].Journal of Agricultural and Food Chemistry, 2018, 66(36):9 556-9 564.
[27] CHUNG C, ROJANASASITHARA T, MUTILANGI W, et al.Enhanced stability of anthocyanin-based color in model beverage systems through whey protein isolate complexation[J].Food Research International, 2015, 76:761-768.
[28] QUAN W, HE W, QIE X J, et al.Effects of β-cyclodextrin, whey protein, and soy protein on the thermal and storage stability of anthocyanins obtained from purple-fleshed sweet potatoes[J].Food Chemistry, 2020, 320:126655.
[29] LANG Y X, LI E H, MENG X J, et al.Protective effects of bovine serum albumin on blueberry anthocyanins under illumination conditions and their mechanism analysis[J].Food Research International, 2019, 122:487-495.
[30] WU J E, GUAN Y G, ZHONG Q X.Yeast mannoproteins improve thermal stability of anthocyanins at pH 7.0[J].Food Chemistry, 2015, 172:121-128.
[31] 赵焕焦, 吕晓玲, 王梦姝, 等.光谱法研究黑米花色苷与酪蛋白的相互作用[J].中国食品添加剂, 2017(1):121-127.ZHAO H J, LV X L, WANG M S, et al.Studies on the interaction between casein and black rice anthocyanin by spectroscopic methodology[J].China Food Additives, 2017(1):121-127.
[32] LI J, WANG B X, HE Y, et al.A review of the interaction between anthocyanins and proteins[J].Food Science and Technology International, 2021, 27(5):470-482.
[33] OANCEA A M, HASAN M, VASILE A M, et al.Functional evaluation of microencapsulated anthocyanins from sour cherries skins extract in whey proteins isolate[J].LWT, 2018, 95:129-134.
[34] KONG F H, KANG S M, AN Y J, et al.The effect of non-covalent interactions of xylitol with whey protein and casein on structure and functionality of protein[J].International Dairy Journal, 2020, 111:104841.
[35] YI J H, QIU M Y, LIU N, et al.Inhibition of lipid and protein oxidation in whey-protein-stabilized emulsions using a natural antioxidant:Black rice anthocyanins[J].Journal of Agricultural and Food Chemistry, 2020, 68(37):10 149-10 156.
[36] JIANG L Z, LIU Y J, LI L, et al.Covalent conjugates of anthocyanins to soy protein:Unravelling their structure features and in vitro gastrointestinal digestion fate[J].Food Research International, 2019, 120:603-609.
[37] ZHANG Y, CHEN S, QI B K, et al.Complexation of thermally-denatured soybean protein isolate with anthocyanins and its effect on the protein structure and in vitro digestibility[J].Food Research International, 2018, 106:619-625.
[38] YANG Y, ZHANG J L, SHEN L H, et al.Inhibition mechanism of diacylated anthocyanins from purple sweet potato (Ipomoea batatas L.) against α-amylase and α-glucosidase[J].Food Chemistry, 2021, 359:129934.
[39] TANG L, LI S, BI H N, et al.Interaction of cyanidin-3-O-glucoside with three proteins[J].Food Chemistry, 2016, 196:550-559.
[40] MENG Y Y, HAO L L, TAN Y, et al.Noncovalent interaction of cyanidin-3-O-glucoside with whey protein isolate and β-lactoglobulin:Focus on fluorescence quenching and antioxidant properties[J].LWT, 2021, 137:110386.
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[1] LI Xiao-min et a. The influence of feruloyl esterase on filterability of wort[J]. Food and Fermentation Industries, 2017, 43(11): 30 -33 .
[2] ZHANG Dong et al. Effect of different amounts of salt on quality of bacon[J]. Food and Fermentation Industries, 2017, 43(11): 159 .
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[5] JI Xiao-kai et al. Research advance in the effect of electric alstimulation on beef quality[J]. Food and Fermentation Industries, 2017, 43(11): 244 .
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[8] . The Absorption of Crosslinked PVP to Tea Polyphenols[J]. Food and Fermentation Industries, 2002, 28(4): 7 .
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