Abstract: This study aimed to analyze the effect of glutamate metabolism on lipid synthesis in the oleaginous filamentous fungus Mortierella alpina. The gene transcription level and enzyme activity of glutamate metabolism-related enzymes and total lipid production in M. alpina under the condition of glutamate supplementation were measured. The results showed that glutamate supplementation significantly regulated the transcription level of glutamate metabolism-related genes in M. alpina. The increased activities of NADP+ glutamate dehydrogenase and glutamate decarboxylase provided sufficient NADPH for the de novo synthesis of fatty acids, which increased the total cell lipid content by 3 g/L (about 10% of the dry weight). This study provides a reference for the analysis of amino acid-mediated microbial lipid synthesis mechanism.
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2022, Vol. 48 
