Abstract: The bacterial community structures and composition differences of three types of Guizhou Douchi, namely dry Douchi, wet Douchi and water Douchi, were investigated and compared by high-throughput sequencing technology. A total of 1 098 891 valid data and 884 bacterial operational taxonomic units (OTUs) were obtained, and clustered into five bacterial phyla and 96 genera. The dominant bacterial phyla were Firmicutes and Proteobacteria, accounting for more than 99.0% in the all three types of Douchi. Bacillus and Proteus were the major genera in dry Douchi, and the dominant genera of wet Douchi were Bacillus and Providencia. The samples of water Douchi were abundant in Bacillus and Tetragonococcus.Bacillus was the dominant genus in the three types of Douchi, and the relative abundance of Bacillus in dry Douchi, wet Douchi and water Douchi were 93.0%, 74.3% and 42.3%, respectively. In addition, the results of cluster analysis and principal component analysis demonstrated that the bacterial communities of dry Douchi and wet Douchi were similar, and the bacterial species of water Douchi were more abundant than others. This study provides a theoretical basis for the standardization and safety management of Guizhou Douchi.
王娜,陈万轩,张伟萍,等. 基于高通量测序技术的贵州不同类型细菌型豆豉菌群多样性分析[J]. 食品与发酵工业, 2022, 48(20): 85-90.
王娜,陈万轩,张伟萍,et al. Community diversity of different bacterial-type of Guizhou Douchi using high throughput sequencing[J]. Food and Fermentation Industries, 2022, 48(20): 85-90.
张蒙冉, 李淑英, 高雅鑫, 等.传统发酵豆制品研究进展[J].食品科技, 2021, 46(1):98-104.ZHANG M R, LI S Y, GAO Y X, et al.Research progress of traditional fermented soybean products[J].Food Science and Technology, 2021, 46(1):98-104.
赵文鹏, 李浩, 杨慧林, 等.曲霉型豆豉发酵阶段细菌群落的演替及其与环境因子的关系[J].食品科学, 2021, 42(4):138-144.ZHAO W P, LI H, YANG H L, et al.Bacterial community succession during the fermentation of Aspergillus-type Douchi, a traditional Chinese fermented soybean product, and its relation with environmental factors[J].Food Science, 2021, 42(4):138-144.
狄飞达, 纪芯钥, 邹强, 等.毛霉型豆豉的研究进展[J].农产品加工, 2021(2):80-83.DI F D, JI X Y, ZOU Q, et al.Research progress on Mucor-fermented soya bean[J].Academic Periodical of Farm Products Processing, 2021(2):80-83.
温嘉敏, 蔡尤林, 黎攀, 等.应用中国根霉12发酵制备高溶栓活性淡豆豉的条件优化[J].食品工业科技, 2018, 39(20):98-104;111.WEN J M, CAI Y L, LI P, et al.Optimization of conditions for using Rhizopus chinesis 12 to ferment and produce semen sojae praeparatum with high-activity fibrinolytic enzyme[J].Science and Technology of Food Industry, 2018, 39(20):98-104;111.
谭小琴. 西南地区传统细菌型豆豉品质特点及微生物群系研究[D].重庆:西南大学, 2021.TAN X Q.Study on the quality characteristics and microbial community of traditional bacterial douchi in Southwest China[D].Chongqing:Southwest University, 2021.
董蕴, 许小玲, 代凯文, 等.基于Miseq高通量测序技术对细菌型豆豉细菌类群的评价[J].食品工业科技, 2018, 39(13):150-154;247.DONG Y, XU X L, DAI K W, et al.Characterization of bacterial microflora of bacteria-fermented Douchi by Miseq high throughput sequencing[J].Science and Technology of Food Industry, 2018, 39(13):150-154;247.
张严峰. 贵州省民间传统水豆豉工业化生产的研究与应用[J].食品与发酵科技, 2016, 52(4):45-48.ZHANG Y F.Guizhou folk traditional natto industrialized production of research and application[J].Food and Fermentation Sciences & Technology, 2016, 52(4):45-48.
张芹. 贵州三穗特色豆豉发酵及保藏技术研究[D].贵阳:贵州大学, 2019.ZHANG Q.Study on the fermentation of Sansui characteristic lobster sauce and preservation technology in Guizhou[D].Guiyang:Guizhou University, 2019.
周芳, 索化夷.永川豆豉发酵过程中抗氧化活性变化及影响因素分析[J].中国调味品, 2019, 44(1):15-19.ZHOU F, SUO H Y.Changes of antioxidant activity and analysis of influencing factors in Yongchuan fermented soybean[J].China Condiments, 2019, 44(1):15-19.
徐慧, 吴晖, 尹志娜, 等.硒纳豆激酶液体发酵研究[J].食品工业科技, 2013, 34(8):219-222;227.XU H, WU H, YIN Z N, et al.Study on the liquid fermentation of Selem' Nattokinase[J].Science and Technology of Food industry, 2013, 34(8):219-222;227.
吉义平, 陈欣, 沈瑞敏.响应面法优化超声辅助提取豆豉低聚糖工艺[J].中国酿造, 2016, 35(5):129-133.JI Y P, CHEN X, SHEN R M.Optimization of ultrasonic-assisted extraction of Douchi oligosaccharides by response surface methodology[J].China Brewing, 2016, 35(5):129-133.
陈怡, 陆敏捷, 刘洋, 等.不同类型豆豉抗氧化活性差异与影响因素分析[J].食品与发酵工业, 2020, 46(13):36-41.CHEN Y, LU M J, LIU Y, et al.Analysis of antioxidant activity of ten kinds of Douchi and their influencing factors[J].Food and Fermentation Industries, 2020, 46(13):36-41.
ZHANG J M, ZHANG C C, WU W C, et al.Correlation of the bacterial communities with umami components, and chemical characteristics in Zhejiang Xuecai and fermented brine[J].Food Research International, 2021, 140:109986.
LUO F P, YANG Z Y, ZHONG K, et al.Effects of Bacillus megaterium L222 on quality and bacterial diversity of Sichuan paocai[J].Food Research International, 2021, 140:109994.
LIN L J, DU F M, ZENG J, et al.Deep insights into fungal diversity in traditional Chinese sour soup by Illumina MiSeq sequencing[J].Food Research International, 2020, 137:109439.
文开勇, 汪月, 文鹏程, 等.四川传统腊肉中微生物群落结构研究[J].食品与发酵工业, 2020, 46(3):36-42.WEN K Y, WANG Y, WEN P C, et al.Study on microbial community structure in Sichuan traditional bacon[J].Food and Fermentation Industries, 2020, 46(3):36-42.
吴成, 王春晓, 王晓丹, 等.高通量测序技术在酿酒微生物多样性研究中的应用[J].食品科学, 2019, 40(3):348-355.WU C, WANG C X, WANG X D, et al.A review of the application of high throughput sequencing technology in analysis of the diversity of microbial communities involved in the fermentation of alcoholic beverages[J].Food Science, 2019, 40(3):348-355.
HU Y Y, WANG H, KONG B H, et al.The succession and correlation of the bacterial community and flavour characteristics of Harbin dry sausages during fermentation[J].LWT, 2021, 138:110689.
DING W W, ZHAO X Y, XIE S, et al.Dynamics and correlation of microbial community and flavor in Pixian Douban fermented with closed process of constant temperature[J].Journal of the Science of Food and Agriculture, 2021, 101(10):4 142-4 153.
聂黔丽, 王修俊, 刘林新, 等.遵义细菌型自然发酵干豆豉菌群结构及风味品质分析[J/OL].食品科学, 2022.http://kns.cnki.net/kcms/detail/11.2206.ts.20210927.2311.038.html.NIE Q L, WANG X J, LIU L X, et al.Analysis on the flora structure and flavor quality of Zunyi naturally fermented dried bacterial-type Douchi[J/OL].Food Science, 2022.http://kns.cnki.net/kcms/detail/11.2206.ts.20210927.2311.038.html.
邓高文, 刘洋, 李跑, 等.基于Illumina MiSeq高通量测序技术分析贵州水豆豉中细菌多样性[J].食品科学, 2021, 42(24):61-66.DENG G W, LIU Y, LI P, et al.Analysis of bacterial diversity in Guizhou Shui-Douchi by Illumina Miseq high-throughput sequencing technology[J].Food Science, 2021, 42(24):61-66.
庞春霞, 李艺, 虞任莹, 等.基于Illumina Miseq技术比较不同地区传统发酵大豆制品细菌多样性[J].食品工业科技, 2022,43(8):133-140.PANG C X, LI Y, YU R Y, et al.Comparison of bacterial diversity of traditional fermented soybean products from different regions based on Illumina Miseq technology[J].Science and Technology of Food Industry, 2022,43(8):133-140.
樊敏, 杨萍, 胡萍, 等.贵州豆豉粑中微生物多样性研究[J].中国酿造, 2019, 38(3):81-88.FAN M, YANG P, HU P, et al.Microbial diversity in Douchiba from Guizhou[J].China Brewing, 2019, 38(3):81-88.