Abstract: As the generation of vinegar residue is increasing, its appropriate disposal is important. Anaerobic digestion is an important way to realize the resourcization and reduction of vinegar residue. A high solid-state continuous anaerobic digestion system was constructed for the vinegar residue treatment by using a full-mixed anaerobic reactor, the biogas generation performance, changes of intermediate metabolites and microbial community structure were investigated under different organic loading rate (OLR). The results indicated that the optimum anaerobic digestion performance was obtained when the organic loading rate reached 6.06 g/(L·d), with the biogas and methane yield of 423 and 226 mL/g, respectively. The relative abundances of Bacteroidetes and Firmicutes were 45.6% and 53.1%, respectively. And the relative abundance distribution of 4 species of microorganism related to lignocellulose metabolism was evenly distributed, which was helpful to the formation of various metabolic pathways to promote substrate hydrolysis. The analysis of archaea showed that Methanobacterium and Methanosarcina were the dominant microorganism, hydrotrophic and mixed-trophic methanogenesis pathways had synergetic effect. When the system was stable, the mass concentrations of total ammonia nitrogen (TAN) and volatile fatty acids (VFAs) were maintained at about 2 and 9 g/L, respectively. High VFAs concentration could inhibit the activity of methanogens and reduce the methane generation rate. Therefore, the fully-mixed high-solid anaerobic digestion system could efficiently treat the vinegar residue. The diversity of microbial community was high, different hydrolysates could be all utilized by methane production pathways in the system.
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