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食品与发酵工业  2022, Vol. 48 Issue (20): 260-264    DOI: 10.13995/j.cnki.11-1802/ts.031216
  分析与检测 本期目录 | 过刊浏览 | 高级检索 |
基于超高效液相色谱-串联质谱技术快速测定果梅中化学成分
夏梦秋1,2, 王星晨1,2, 杨兰香1,2, 岳婧怡1,2, 王瑞1,2, 曹侃1,2, 程旺开1,2*
1(芜湖职业技术学院,安徽 芜湖,241000)
2(芜湖生命健康工程技术研究中心,安徽 芜湖,241000)
Rapid determination of chemical components in fruit plum based on ultra performance liquid chromatography-tandem mass spectrometry technique
XIA Mengqiu1,2, WANG Xingchen1,2, YANG Lanxiang1,2, YUE Jingyi1,2, WANG Rui1,2, CAO Kan1,2, CHENG Wangkai1,2*
1(Wuhu Institute of Technology, Wuhu 241000, China)
2(Wuhu Life and Health Engineering and Technology Research Center, Wuhu 241000, China)
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摘要 采用超高效液相色谱-串联质谱技术对果梅的化学成分进行快速测定。采用ZORBAX SB-C18(3.0 mm×100 mm,3.5 μm)色谱柱,流动相为水(含体积分数0.2%甲酸)(A)和乙腈(含体积分数0.2%甲酸)(B),采用梯度洗脱方式,柱温30 ℃,流速为0.2 m/min, 进样量为1 μL。从果梅中成功测定了16种化学成分,主要包括10种有机酸、4种黄酮类、5-羟甲基糠醛和苦杏仁苷。该文成功快速测定了果梅中主要的化学成分,为进一步研究果梅的药效基础和作用机制提供理论基础。
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夏梦秋
王星晨
杨兰香
岳婧怡
王瑞
曹侃
程旺开
关键词:  果梅  超高效液相色谱-串联质谱法  化学成分    
Abstract: ultra performance liquid chromatography-tandem mass spectrometry was used to determine the chemical components in fruit plum. The analysis conditions were as follows. The chromatographic separation was performed using a ZORBAX SB-C18 (3.0 mm×100 mm, 3.5 μm) column, and the temperature of the column was maintained at 35 ℃. The mobile phases were 0.2% formic acid (A) and acetonitrile (including 0.2% formic acid) (B), respectively, using a binary gradient elution at the flow rate of 0.2 mL/min. Results showed that sixteen chemical components were successfully determined in fruit plum, including 10 organic acids, 4 flavonoids, 5-hydroxymethylfurfural, and bitter amygdidine. The main chemical compositions of fruit plum were successfully and quickly determined in this paper and the results could provide the theoretical basis for further study of the efficacy basis and mechanism of action.
Key words:  fruit plum    ultra performance liquid chromatography-tandem mass spectrometry    chemical composition
收稿日期:  2022-02-26      修回日期:  2022-05-20           出版日期:  2022-10-25      发布日期:  2022-11-18      期的出版日期:  2022-10-25
基金资助: 2022年度校级科学研究项目(wzyzr202231);2019年安徽省教育厅质量工程项目(皖教秘高[2019]94号;2019cxtd023)
作者简介:  硕士研究生(程旺开教授为通信作者,E-mail:448408718@qq.com)
引用本文:    
夏梦秋,王星晨,杨兰香,等. 基于超高效液相色谱-串联质谱技术快速测定果梅中化学成分[J]. 食品与发酵工业, 2022, 48(20): 260-264.
夏梦秋,王星晨,杨兰香,et al. Rapid determination of chemical components in fruit plum based on ultra performance liquid chromatography-tandem mass spectrometry technique[J]. Food and Fermentation Industries, 2022, 48(20): 260-264.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.031216  或          http://sf1970.cnif.cn/CN/Y2022/V48/I20/260
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