Abstract: ultra performance liquid chromatography-tandem mass spectrometry was used to determine the chemical components in fruit plum. The analysis conditions were as follows. The chromatographic separation was performed using a ZORBAX SB-C18 (3.0 mm×100 mm, 3.5 μm) column, and the temperature of the column was maintained at 35 ℃. The mobile phases were 0.2% formic acid (A) and acetonitrile (including 0.2% formic acid) (B), respectively, using a binary gradient elution at the flow rate of 0.2 mL/min. Results showed that sixteen chemical components were successfully determined in fruit plum, including 10 organic acids, 4 flavonoids, 5-hydroxymethylfurfural, and bitter amygdidine. The main chemical compositions of fruit plum were successfully and quickly determined in this paper and the results could provide the theoretical basis for further study of the efficacy basis and mechanism of action.
夏梦秋,王星晨,杨兰香,等. 基于超高效液相色谱-串联质谱技术快速测定果梅中化学成分[J]. 食品与发酵工业, 2022, 48(20): 260-264.
夏梦秋,王星晨,杨兰香,et al. Rapid determination of chemical components in fruit plum based on ultra performance liquid chromatography-tandem mass spectrometry technique[J]. Food and Fermentation Industries, 2022, 48(20): 260-264.
张永春,包满珠.梅树品种分类研究进展[J].北京林业大学学报,1998, 20(2):90-94.ZHANG Y C, BAO M Z.Advances in classification for cultivars of Prunus mume[J].Journal of Beijing Forestry University, 1998,20(2):90-94.
[2]
刘兴艳,蒲彪,刘云,等.大邑果梅基础营养成分含量的测定和研究[J].食品研究与开发, 2007,28(6):146-148.LIU X Y, PU B, LIU Y, et al.Determination of the basic nutritional ingredient of plums (Prunus mume Sieb.et Zucc) in Dayi[J].Food Research and Development, 2007, 28(6):146-148.
[3]
潘惠慧,吴晓琴,陆柏益,等.高效液相色谱法分析和检测青梅花、枝、叶中有机酸的种类和含量[J].科技通报,2008,24(3):350-354.PAN H H, WU X Q, LU B Y, et al.Separation and determination of organic acids in flower, branch and leaf extract of Prunus mume by HPLC[J].Bulletin of Science and Technology, 2008,24 (3):350-354.
[4]
陈战国,恩伯提,张志琪.RP-HPLC同时测定乌梅中8种有机酸含量[J].中国中药杂志,2006, 31(21):1 783-1 786.CHEN Z G, EN B T, ZHANG Z Q.Simultaneous determination of eight organic acids in Fructus Mume by RP-HPLC[J].China Journal of Chinese Materia Medica, 2006, 31(21):1 783-1 786.
[5]
安苗,黎雄,赵亚,等.HPLC-PDA同时测定乌梅肉中3种特征成分[J].中国实验方剂学杂志,2017,23(23):52-56.AN M, LI X, ZHAO Y, et al.Simultaneous determination of three main components in mume fructus pulp by HPLC-PDA[J].Chinese Journal of Experimental Traditional Medical Formulae, 2017,23(23):52-56.
[6]
周茜,韩雪,韩晓梅,等.响应面试验优化乌梅熊果酸提取工艺及其对大肠杆菌的抑制作用[J].食品科学,2016,37(8):67-73.ZHOU Q, HAN X, HAN X M, et al.Optimization of ursolic acid extraction from fructus mume and evaluation of its antibacterial activity against Escherichia coli[J].Food Science, 2016,37 (8):67-73.
[7]
宋爽. 梅果实提取液抑菌、抗氧化活性成分鉴定与功能分析[D].南京:南京农业大学,2016.SONG S.Identification and function analysis of Japanese apricot fruit extraction's bacteriostatic and antioxidative active ingredient[D].Nanjing:Nanjing Agricultural University, 2016.
[8]
张飞,李劲松.果梅的研究进展[J].海峡药学,2006(4):21-24.ZHANG F, LI J S.Research progress in Umei [J].Straits Pharmacy, 2006(4):21-24.
[9]
刘友平,陈鸿平,万德光,等.乌梅的研究进展[J].中药材,2004(6):459-462.LIU Y P, CHEN H P, WAN D G, et al.Research progress in Umei [J].Journal of Chinese Medicinal Materials, 2004 (6):459-462.
[10]
李昕,王瑞,李肖莉,等.不同加工方法乌梅UPLC特征图谱及模式识别研究[J].中国中医药信息杂志,2020,27(11):76-81.LI X, WANG R, LI X L, et al.Study on UPLC fingerprints and pattern recognition of mume fructus with different processing methods[J].Chinese Journal of Information on Traditional Chinese Medicine, 2020,27 (11):76-81.
[11]
OU J M, WANG R, LI X L, et al.Comparative analysis of free amino acids and nucleosides in different varieties of mume fructus based on simultaneous determination and multivariate statistical analyses.[J].International Journal of Analytical Chemistry, 2020,2020:4767605.
[12]
甘源,贺丽迎,赵舰,等.超高效液相色谱高分辨质谱法同时测定蜂蜜中的5种雷公藤类物质[J].中国卫生检验杂志,2021,31(6):650-653;658.GAN Y, HE L Y, ZHAO J, et al.Simultaneous determination of 5 kinds of Tripterygium substances in honey by liquid chromatography high resolution mass spectrometry[J].Chinese Journal of Health Laboratory Technology, 2021, 31(6):650-653;658.
2022, Vol. 48 
