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食品与发酵工业  2022, Vol. 48 Issue (20): 122-129    DOI: 10.13995/j.cnki.11-1802/ts.032698
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
金抗肽SIF4基于糖代谢途径和细胞质膜氧化损伤的大肠埃希菌抑菌机理
李玉珍1, 肖怀秋1,2*, 王琳1, 刘淼1, 曾梦琪1, 曹丹1, 赵谋明2
1(湖南化工职业技术学院 制药与生物工程学院,湖南 株州,412000)
2(华南理工大学 食品科学与工程学院,广东 广州,510000)
Antimicrobial mechanism of metal antimicrobial peptide SIF4 against Escherichia coli based on glucose metabolism pathway and cytoplasmic membrane oxidative damage
LI Yuzhen1, XIAO Huaiqiu1,2*, WANG Lin1, LIU Miao1, ZENG Mengqi1, CAO Dan1, ZHAO Mouming2
1(School of Pharmaceutical and Bioengineering, Hunan Chemical Vocational Technology College, Zhuzhou 412000, China)
2(School of Food Science and Engineering, South China University of Technology, Guangzhou 510000, China)
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摘要 为揭示金抗肽SIF4基于糖代谢途径和细胞质膜氧化损伤的大肠埃希菌抑菌机理,研究了SIF4对糖酵解和三羧酸循环途径关键酶活性、细胞质膜氧化损伤及细胞内源活性氧自由基(reactive oxygen species,ROS)生成的影响。研究发现,SIF4对己糖激酶、磷酸果糖激酶和丙酮酸激酶这3种糖酵解关键酶抑制率分别达66.61%、27.37%和39.61%,对丙酮酸脱氢酶系、柠檬酸合成酶、异柠檬酸脱氢酶和α-酮戊二酸脱氢酶这4种三羧酸循环关键酶抑制率分别达25.20%、22.91%、26.99%和23.44%,主要通过抑制大肠埃希菌糖酵解途径中已糖激酶活性来调控葡萄糖氧化效率而发挥抑菌活性。研究还发现,SIF4可造成细胞质膜氧化损伤和诱导氧化应激产生过量ROS,破坏细胞质膜结构与功能,从而诱导菌体细胞凋亡或程序性死亡来发挥协同抑菌活性。试验结果可为系统揭示SIF4对大肠埃希菌抑菌机理提供直接证据,也为其在食源性大肠埃希菌生物防控中的应用提供理论支持。
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李玉珍
肖怀秋
王琳
刘淼
曾梦琪
曹丹
赵谋明
关键词:  金抗肽  大肠埃希菌  糖酵解途径  三羧酸循环  细胞质膜氧化损伤    
Abstract: To reveal the antimicrobial mechanism of metal antimicrobial peptide (MAP) SIF4 against Escherichia coli based on glucose metabolism pathway and cytoplasmic membrane oxidative damage, the effects of SIF4 on critical enzymatic activity of Embden-Meyerhof-Parnas (EMP) pathway and tricarboxylic acid (TCA) cycle, cytoplasmic membrane oxidative damage, intracellular ROS production and cell membrane depolarization were investigated. Results showed that, SIF4 inhibited the key enzymes in glycolysis, including hexokinase (HK), phosphofructokinase (PFK) and pyruvate kinase (PK), by 66.61%, 27.37, and 39.61%, respectively, and the key enzymes in TCA cycle, including pyruvate dehydrogenase complex (PDHC), citrate synthetase (CS), isocitrate dehydrogenase (IDH) and α-ketoglutaric dehydrogenase (α-KGDH), by 25.20%, 22.91%, 26.99 and 23.44%, respectively. SIF4 exerted good antimicrobial capacity mainly through regulating glucose oxidation efficiency by restraining the HK activity in glycolysis. It was also confirmed that SIF4 could cause cytoplasmic membrane oxidative damage and induce oxidative stress to produce excessive ROS, resulting in destroying cytoplasmic membrane structural integrity, and exerting synergistic enhancement of antimicrobial capacity by inducing cell apoptosis or programmed cell death. All results can provide direct evidence for systematically elucidating antimicrobial mechanism of SIF4 against E. coli, and also offer theoretical support for its application in the biological control of foodborne E. coli.
Key words:  metal antimicrobial peptide    Escherichia coli    EMP pathway    TCA cycle    cytoplasmic membrane oxidative damage
收稿日期:  2022-06-16      修回日期:  2022-06-30           出版日期:  2022-10-25      发布日期:  2022-11-18      期的出版日期:  2022-10-25
基金资助: 湖南省自然科学基金科教联合基金(2022JJ60046)
作者简介:  硕士,副教授(肖怀秋教授为通信作者,E-mail:xiaohuaiqiu@163.com)
引用本文:    
李玉珍,肖怀秋,王琳,等. 金抗肽SIF4基于糖代谢途径和细胞质膜氧化损伤的大肠埃希菌抑菌机理[J]. 食品与发酵工业, 2022, 48(20): 122-129.
李玉珍,肖怀秋,王琳,et al. Antimicrobial mechanism of metal antimicrobial peptide SIF4 against Escherichia coli based on glucose metabolism pathway and cytoplasmic membrane oxidative damage[J]. Food and Fermentation Industries, 2022, 48(20): 122-129.
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