为研究果胶对牙龈卟啉单胞菌(Porphyromonas gingivalis, Pg)加剧的结肠炎的干预效果,将小鼠随机分为3组:模型组、Pg处理组、果胶治疗组。在1~7 d,各组小鼠给予2%葡聚糖硫酸钠(dextran sodium sulphate,DSS),建立DSS诱导的结肠炎小鼠模型。在1~14 d,Pg处理组和果胶治疗组小鼠灌胃1×108 CFU Pg。此外,连续14 d给予果胶组小鼠200 mg/kg果胶。检测各组小鼠的体重变化、脾脏指数、结肠长度、脾脏免疫T细胞情况、结肠病理组织、血清细胞因子水平及肠道菌群。研究结果显示,Pg能加剧小鼠结肠炎症状,包括体重减轻、结肠长度缩短、肠道组织受损严重加剧,进一步实验发现小鼠脾脏免疫Th17细胞增加、血清细胞因子水平增加,肠道菌群紊乱。果胶处理后,缓解了小鼠的结肠炎症状,减轻了体重下降、结肠缩短和肠道组织的损伤,并降低了血清中细胞因子水平,缓解了肠道菌群紊乱。
Abstract
To investigate the intervention effect of pectin on exacerbated colitis induced by Porphyromonas gingivalis (Pg), mice were randomly assigned into three groups, including model group, Pg treatment group, and pectin treatment group.From days 1 to 7, all groups of mice were administered 2% DSS to establish a DSS-induced colitis mouse model.From days 1 to 14, mice in the Pg group and pectin group were orally gavaged with 1×108 CFU Pg.Additionally, mice in the pectin group received continuous pectin treatment at a dose of 200 mg/kg for 14 days.The body weight, spleen index, colon length, splenic immune T-cell status, colonic histopathology, serum cytokine levels, and intestinal microbiota of each group of mice were examined.Results showed that Pg exacerbated colitis symptoms in mice, including body weight loss, colon shortening and exacerbated colonic tissue damage.Further experiments revealed increased splenic immune Th17 cells, elevated serum cytokine levels, and disrupted gut microbiota.Treatment with pectin alleviated colitis symptoms in mice, attenuating weight loss, colon shortening, and intestinal tissue damage, while also reducing serum cytokine levels and ameliorating disruption of gut microbiota.
关键词
果胶 /
牙龈卟啉单胞菌 /
结肠炎 /
Th17 /
肠道菌群
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Key words
pectin /
Porphyromonas gingivalis /
colitis /
Th17 /
gut microbiota
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脚注
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基金
国家自然科学基金项目(31970088);科技部国家重点技术研究发展计划项目(2020YFA0509600)
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