Abstract: Cellulase can be induced by different inducers. A desirable carbon source should support the growth of microorganisms and induce the expression of cellulase genes effectively. Self-made sugar mixture obtained by transglycosylation from glucose (F carbon source),microcrystalline cellulose (C carbon source) and the multiple carbon source (M carbon source,mixture of F and C in the ratio 1:2) were applied in the submerged fermentation of cellulase with Trichoderma Reesei ZU-04. Properties of carbon sources were found to have different effects on the cellulase components. On F carbon source,the enzyme activities of endo-β-1,4-glucanase (Cx),exo-β-1,4-glucanase (C1) increased quickly and reached their peak value at around 40 h,but the activity of (-glucosidase(CB) were remained extremely low throughout the process. On C carbon source,the activities of Cx,C1 and CB grew slowly at the initial stage,but the production cycle sustained longer with the continuous increase of enzyme activities. M carbon source could both accelerate the formation rate and enhance the production sustainability of Cx and C1,Cx activities increased by 78.2% and 51.9%,and C1 activities increased by 20.6% and 6.5% compared with those produced by C and F carbon sources respectively. As to the β-glucosidase production,the F carbon source was the worst among all,and C carbon source was better than M carbon source. The influence of different carbon sources on filter paper activities was further studied. F carbon source produced the highest filter paper activity of 1.60 IU/mL within 36 h,featured by a rapid celluase formation rate,yet unsustainable production cycle. Filter paper activity induced by C carbon source reached a maximum of 3.96 IU/mL within 144 h,yet,cellulase activity increased slowly at the initial stage and cellulase production cycle lasted longer. Combined the advantages of F and C,M carbon source could maintain both filter paper activity and productivity at relatively high levels. The results are of great significance in improvement of cellulase productivity and regulation of cellulase system.
吕雄,赵晶,夏黎明. 碳源对里氏木霉纤维素酶诱导合成的影响[J]. 食品与发酵工业, 2010, 36(03): 1-4.
Lv Xiong,Zhao Jing,Xia Liming. Effects of Different Carbon Sources on Cellulase Production by Trichoderma reesei[J]. Food and Fermentation Industries, 2010, 36(03): 1-4.