Abstract: The effect of antioxidant in respect of preventing diseases and delaying aging has been paid attention. Searching better and higher effective antioxidant was even more important. In this article,antioxidant activity of 26 plants samples were studied by DPPH method,and DPPH free radical scavenging capability of extracts were determined. Experimental results showed that the antioxidant components were soluble in alcoholic solution but not in water; among these 26 plants,Radix sanguisorbae,Caulis spatholobi,Radix paeoniae Rubra and Polygonum cuspidate had higher antioxidant activities than other plants. Radix sanguisorbae was the highest with IC50 of 8.89 μg/mL.