Abstract: To explore the technology of extracting polysaccharide from the leaves of the Hippohae rhamnoides L.,we studied the ratio of the polysaccharides extraction by using three single factors,that is,the ratio between the liquids and the raw materials,the power of the supersonic and the acting time of the supersonic.Using the analytical method of the response surface,we found that under the following condition: liquids to raw materials ratio 52.5∶1,supersonic power of 607.1 W,supersonic acting time of 42.3 minutes,as much as 7.50% of the polysaccharide can be obtained.After initial purification of the polysaccharide,we conducted the bacteriostasis experiments using the agar plate dilution method.The results showed that the polysaccharide from the leaves of the Hippohae rhamnoides L.had the certain inhibiting effects on some pathogenic bacteriums such as the Escherichia coli and the Staphylococcus aureus.
包怡红,秦蕾. 沙棘叶多糖的超声波辅助提取及抑菌作用研究[J]. 食品与发酵工业, 2010, 36(05): 161-166.
Bao Yi-hong,Qin Lei. Study on Supersonic Wave-assisted Extraction and Bacteriostatic of the Polysaccharide from the Leave in Hippohae rhamnoides L.[J]. Food and Fermentation Industries, 2010, 36(05): 161-166.