Abstract: Space mutation was carried out with an exopolysaccharide(EPS)-producing strain,Lactobacillus casei LC2W,by spaceship carried experiment of manned spacecraft Shenzhou-7.Three high-yielding EPS strains,LC2W-1,LC2W-2 and LC2W-3,had been screened out and also showed good genetic stability after cultivated for 10 generations.The fermentation conditions of LC2W-1 were optimized by means of single factor tests and orthogonal tests.The optimum conditions for synthesis of EPS by LC2W-1 were as follows: adding 4% glucose and 0.6% yeast extract into the skimmed milk medium and cultured at 33℃for 26h.Under these conditions,the yield of EPS could reach(276.76±7.34) mg/L,which was increased by 44.54% and 104.93% compared with that produced by the mutant and original strain,respectively.
Zhou Fang-fang,Wu Zheng-jun,Chen Chen The Technology Center of Bright Dairy & Food Co.,等. 太空搭载高产胞外多糖乳酸菌菌株的选育及其发酵条件优化[J]. 食品与发酵工业, 2011, 37(06): 35-38.
周方方,吴正钧,陈臣. Screening of Mutant with High Production of Exopolysaccharide after Spaceship-carried Experiment and Optimization of Its Fermentation Condition[J]. Food and Fermentation Industries, 2011, 37(06): 35-38.