Abstract: Multiplex realtime PCR plays a great role in foodborne pathogens detection,and internal amplification control(IAC) is necessary for indicating the possible false negative detection results.An IAC was constructed based on an DNA sequence originated from human Adenovirus,and its detection specificity,amplification efficiency and antijamming capability against target pathogens were evaluated in present study.As shown in results,there is no non-specific amplification result by primer of IAC,and an as high as 99.44% of amplification efficiency was obtained.Furthermore,the IAC in real time PCR system showed no interference on the amplifications of tested Salmonella,Escherichia coli O157:H7 and Lsteria monocytogenes strains,and present a broad prospect of application in multiplex foodborne pathogens detections.
索标,滕要辉,艾志录,等. 食源性致病菌多重实时荧光PCR检测扩增内标的构建及评价[J]. 食品与发酵工业, 2011, 37(08): 148-151.
Suo Biao,Teng Yao-hui,Ai Zhi-lu,et al. Construction and Evaluation of an Internal Amplification Control for Multiplex Realtime PCR Detection of Foodborne Pathogens[J]. Food and Fermentation Industries, 2011, 37(08): 148-151.