Abstract: With the badanmu protein as the substrate,the orthogonal test was taken to study the enzymatic hydrolysis condition of alcalase enzyme on badanmu proteins.The reducing power and the scavenging activity to hydroxyl,superoxide radical,and DPPH· free radicals were also investigated.The optimum hydrolysis parameters were as follows: substrate concentration 20%,enzyme concentration 4%,pH value 9.5,temperature 60℃.In this condition of hydrolysis,its hydrolysis degree for badanmu protein is 36.6%.The reductive ability of badanmu protein peptide increased with peptide concentration increasing.The protein peptide also had good OH·,O2-·,and DPPH· scavenging activity,which increased with the concentration of peptides until its peak.The antioxidation activity of badanmu peptides were almost fixed when its concentration exceeded a certain level.
孙月娥,王卫东,郑义. 巴旦木蛋白水解物的制备及其抗氧化活性[J]. 食品与发酵工业, 2011, 37(10): 105-109.
Sun Yue-e,Wang Wei-dong,Zheng Yi. Study on Preparation and Antioxidant Activity of Hydrolysate from Badanmu Protein[J]. Food and Fermentation Industries, 2011, 37(10): 105-109.