Abstract: The quantitative detection of Zearalenone(ZEN) was investigated on the microsphere array technology platform and indirect competition theory.In this research,Monoclonal Anti-ZEN was chosen and labeled by biotin.ZEN-BSA was coupled with 36﹟microsphere,then it was added in the 1.5mL centrifuge tube with the free target antigen and the biotin labeled Monoclonal Anti-ZEN.Botin labeled Monoclonal Anti-ZEN was competitive bound by the coupling antigen and the free target antigen,and the target antigen was detected by the report molecule SA-PE which was stimulated fluorescence at 532nm laser light.The fluorescence intensity was inversely proportional to the concentration of the free target antigen.Through optimizing the concentration of the critical saturated Monoclonal Anti-ZEN and determining the incubation time of antigen and antibody,the quantitative detection of ZEN was established eventually.In this research,the critical saturated concentration of Monoclonal Anti-ZEN was 2.0 μg/mL,the best incubation time of antigen and antibody was 3h.As referring of the international current standard of IC50,the results showed that the sensitivity was 0.0054 μg/mL,the detection range of the standard curve was 0.0008~0.04 μg/mL.By applying the research method in skim milk and full milk contamination testing,the recovery rate of the two kinds of milk was both greater than 75%.The procedure of purification and separation of the sample were simplified by using this method.Overall,the quantitative analysis of ZEN using the microsphere array technology is sensitive,stable,rapid,and simple,and it is applicable for mass sample testing.
宋慧君,蒋丹,马惠蕊,等. 一种新型玉米赤霉烯酮的定量检测方法[J]. 食品与发酵工业, 2011, 37(10): 149-153.
Song Hui-jun,Jiang Dan,Ma Hui-rui,et al. A New Method for the Zearalenone Quantitative Detection[J]. Food and Fermentation Industries, 2011, 37(10): 149-153.
2011, Vol. 37 
