Abstract: In this paper,microwave is used to bake tea seed kernel,and the optimal parameter of tea seed oil extraction process by supercritical CO2 is studied.Experimental results show that the optimal conditions are:extraction temperature 60℃,extraction pressure 30MPa,extraction time 100 min,CO2 flow rate 45~55 kg/h,and the extraction rate is 94.1%.The physical and chemical properties and the fatty acid composition of the tea seed oil are determined.