Abstract: In order to provide reference for industrialized production of DHA,the effect of fermentation mode on the growth of C. cohnii and accumulation of DHA was studied. After optimization of the initial concentration of glucose during batch fermentation,we got the maximal DHA production when the concentration of glucose was 60g / L. Under this condition,the DHA yield reached 0. 8g / L after cultivating for 9 days. Based on the research of batch fermentation,30g glucose per liter of medium was fed once during stationary phase,the DHA yield reached 1. 27g / L after cultivating for 9 days. What's more,when glucose was fed once every 24 hours to keep the concentration of glucose at20g / L,the DHA yield reached 1. 72g / L after cultivating for 9 days. Results showed that fed-batch fermentation was superior to batch fermentation in the accumulation of DHA in C. cohnii.
肖尚,孙立洁,姚黎明,等. 补料分批发酵对隐甲藻生长和积累DHA的影响[J]. 食品与发酵工业, 2013, 39(09): 31-35.
XIAO Shang,SUN Li-jie,YAO Li-min,et al. The effect of fed-batch fermentation on growth of C. cohnii and accumulation of DHA[J]. Food and Fermentation Industries, 2013, 39(09): 31-35.