Abstract: According to the screening standards of meat fermentation starter,four lactic acid bacteria strains( L4,L12,L20 and L36) with excellent characteristics were isolated and screened from salted and fermented manarin fish. And the results of traditional physiological and biochemical identification and 16S rDNA sequence analysis showed that the four strains were Enterococcus faecium,Lactobacillus curvatus,Enterococcus durans,and Lactobacillus casei respectively.