Abstract: For optimizing the technological conditions of producing trehalose synthase from E. coli BL21 after permeated by colistin sulfate,a novel visualized method for the operation of industrial process called visualized optimization method was adapted in this paper. The results indicated that a proper process was achieved with cell suspension treated by 1. 40 g /L colistin sulfate for 72 min at 35℃ and the obtained enzyme activity was 2. 09 times higher than that from traditional method. Utilizing the permeabilized cells for batch fermentation,the maximum conversion rate could reach 70% with 30% maltose as the substrate. The cells could be also used for at least 16 batches( 12 h per batch),and the conversion rate kept above 65%.
崔怀言,江凌,张文婧,等. 采用可视化方法通透性处理大肠杆菌细胞生产海藻糖合成酶[J]. 食品与发酵工业, 2014, 40(06): 8-12.
CUI Huai-yan,JIANG Ling,ZHANG Wen-jing,et al. Optimization of permeability of E. coli cells in the production of trehalose synthase by a visualized method[J]. Food and Fermentation Industries, 2014, 40(06): 8-12.