Abstract: In order to measure the degradation rate of cholesterol of five strains in vitro,o-phthalalehpyde( OPA)method and High performance liquid chromatography( HPLC) method were used to determine the cholesterol of the medium respectively. Linearity,precision and recovery between the two methods were compared. The results were as follows:the degradation rate of cholesterol of five strains are among 12. 34% ~ 28. 57%,two methods both had good linearity in the rang of 0 ~ 0. 20 mg /mL and 0 ~ 0. 70 mg /mL respectively,precision of relative standard deviation was 0. 32%( n =5) and 0. 96%( n = 5),the spike recoveries were both between 99. 33% and 100. 18%. Overall comparison indicated that both the OPA method and HPLC method can measure the degradation rate of cholesterol of bifidobacteria.
张汝娇,何腊平,李翠芹,等. 邻苯二甲醛法(OPA)与高效液相色谱法(HPLC)测定降胆固醇的双歧杆菌的对比[J]. 食品与发酵工业, 2014, 40(07): 177-181.
ZHANG Ru-jiao,HE La-ping,LI Cui-qin,et al. The comparison of o-phthalalehpyde(OPA) method and high performance liquid chromatography(HPLC) in the determination of cholesterol-lowering bifidobacteria[J]. Food and Fermentation Industries, 2014, 40(07): 177-181.