Abstract: The best condition for water extraction of dendrocalamus latiflorus bamboo polysaccharides was as follows: the ratio of solid to liquid was 40∶ 1,the final alcohol concentration was 80%,the temperature was 80℃,and the time was 1hour. Under this condition,bamboo shoots polysaccharide extraction rate was 2. 19%. Extraction of bamboo shoots polysaccharide assisted by Single enzyme( pectinase,cellulase,neutral protease) was studied using recommended optimum hydrolysis conditions,wherein extraction assisted by neutral protease reached the highest rate.The neutral protease-assisted extraction of dendrocalamus latiflorus bamboo polysaccharides was optimized by single factor variation method and the optimum enzymatic conditions were found as follows: hydrolysis 2 times,the enzyme dosage of 3. 0%,at 45 ℃,pH value was 4. 6. Under these conditions,after enzymatic hydrolysis for 60 min combined with water extraction and alcohol precipitation,the extraction rate reached 2. 91%,which was improved by32. 9% compared with the traditional method of water extraction and ethanol precipitation.