利用Minitab软件,采用PB设计,选择温度、pH、接菌量、转速、初始加油量5个影响因素,对从延安川口受石油污染土壤中分离筛选得到的可降解石油菌株CT-6的降解条件进行筛选优化,结果为:当温度为39.6℃,pH为7.0,转速为198 r/min,接菌置为8%,初始加油量为2.9%时,菌株对石油降解率比优化之前提高了28.3%,达到89.4%.

The experiment which used minitab software and applied Packett-Burman design selected five factors: temperature, pH, inoculate capacity, rotational speed and the initial amount of oil to optimize the degradation condi-tion of oil degrading strain CT-6 which was isolated and screened from the soil polluted by oil of Chuankou in Yan'an.The result showed as follows:when the temperature was 39.6℃, pH 7.0, inoculate capacity 8%, rotational speed 198 r/min, the original amount of oil 2.9% , the strain CT-6 made the rate of oil degradating increase by 28.3%,which reached 89.4%.