Determination of uniconazole and storage stability in Zanthoxylum bungeanum Maxim by QuEChERS-UHPLC-MS/MS
FAN Jinping1, ZHANG Ying2, WEI Jin2*, LUO Shixia1, DUAN Tingting2*
1(College of chemistry and materials science, Guizhou Normal University, Guiyang 550025,China) 2(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550009,China)
Abstract: A method for the simultaneous determination of uniconazole in Zanthoxylum bungeanum Maxim by ultra performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was established. The experimental samples were extracted with acetonitrile containing 0.1% acetic acid and purified with PSA and GCB. The mobile phase was eluted with acetonitrile and 0.1% formic acid aqueous solution. The analytes were separated on an Hypersil GOLD C18 column and quantitatively analyzed by the external standard method using matrix-matched calibration curves. The results showed that there was good linearity in the range of 1.0-50 ng/mL under the optimal conditions, with correlation coefficients R2 of 0.993 7-0.999 7. The spiked recoveries of uniconazole in Zanthoxylum bungeanum Maxim at three spiked levels of 0.02, 0.05, 0.20 mg/kg ranged from 71.50% to 85.98%, with relative standard deviations RSDs of 1.13%-5.36%. The limits of quantitation of LOQ were 0.02 mg/kg. Moreover, the average degradation rate of uniconazole in Zanthoxylum bungeanum Maxim was less than 30% at -20 ℃. The established method was simple, sensitive, accurate and reliable, and could meet the requirement of multi pesticide residues analysis in mango skin and flesh. It was of great significance to solve the safety problems for the residue of uniconazole in foods.
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FAN Jinping,ZHANG Ying,WEI Jin,et al. Determination of uniconazole and storage stability in Zanthoxylum bungeanum Maxim by QuEChERS-UHPLC-MS/MS[J]. Food and Fermentation Industries, 2021, 47(8): 230-235.
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2021, Vol. 47 
