Abstract: Donkey-hide gelatin and bovine-hide gelatin were determined in 6 kinds of formulations, such as powder, capsule, lozenge, jell, and oral solution, and 67 samples of donkey-hide gelatin using UPLC-MS/MS. The contents of donkey-hide gelatin and bovine-hide gelatin were analysed and evaluated by measuring L-hydroxyproline, glycine, alanine, L-proline, moisture, and protein. The content of donkey-hide gelatin in tablets was found to be 39%-65%, and protein was 68%-84%. The detection rate of bovine-hide gelatin was 22.8%, and 2 batches exceeded the standard. The content of donkey-hide gelatin indicated as the sum of L-hydroxyproline, glycine, alanine, and L-proline had significant differences in other 5 formulations, as they were all lower than that in tablets. Moreover, 16 batches were detected to contain bovine-hide gelatin, but none of them exceeded the standard. The present method is repeatable and sensitive to distinguish donkey-hide gelatin and bovine-hide gelatin. It is necessary to pay attention to raw materials of donkey-hide gelatin and formulate standards for different formulations.
王超,迟少云,杨钊,等. 阿胶类保健食品中阿胶成分和牛皮源成分检测及质量评价[J]. 食品与发酵工业, 2019, 45(1): 224-228.
WANG Chao,CHI Shaoyun,YANG Zhao,et al. Quality evaluation and determination of donkey-hide gelatin and bovine-hide gelatin in health-care food of donkey-hide gelatin[J]. Food and Fermentation Industries, 2019, 45(1): 224-228.