1(Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China) 2(Beijing Wuzi University, School of Logistics/Beijing Key Laboratory of Logistics System and Technology, Beijing 101149, China)
Abstract: In order to identify the role of gibberellins (GAs) in the resistance of tomato fruits, the present study used the wild type (WT) and GA-deficient mutant (gib-3) tomato fruits at the mature green stage as raw materials and stored them at 4 ℃. The chilling injury (CI) index was measured, the ultra-structure of cells was observed, cell membrane damage intensity, as well as the activity changes of cell membrane lipase, cell wall degradation enzymes, and antioxidant enzymes of tomato fruits were determined. Results indicated that gib-3 tomato fruits showed more severe chilling symptoms and ultra-structural damage than WT tomato fruits. The MDA content, ion permeability and the activity of PLD, LOX, PG and PME of gib-3 tomato were increased. Cell membrane peroxidation and cell wall degradation of gib-3 tomato fruits accelerated, and their integrity of fruit cell membrane and cell wall was damaged. In addition, the activity of SOD, CAT, PAL and GST were decreased in gib-3 tomato fruits, and the ability of free radical scavenging reduced. According to the results, gibberellin deficiency at low temperatures was not good for tomato fruits to resist chilling injury. This study will provide a theoretical basis for the research on the chilling resistance mechanism of postharvest tomato fruits.
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