Abstract: Response surface experiment was designed to identify optimum medium. The optimum medium for Bacillus amyloliquefaciens L536 was composed of:NaCl 15 g/L,soybean meal 53 g/L,sodium citrate 16 g/L,sodium hydrogen glutamate 40 g/L,NH4Cl 0.46 g/L,K2HPO4 0.625 g/L,MgSO4·7H2O 1.25 g/L,MnSO4·H2O 0.35 g/L,CaCl2·2H2O 0.2 g/L. Under these conditions,the yield of γ-PGA was 25.81 g/L,increased for about 1.5 times more than before. In order to obtain the optimum fermentation medium for γ-PGA production,the method of response surface experimental design,experimental procedure and results are discussed in this paper.
李文婧,赵祥颖,田延军,等. γ-聚谷氨酸产生菌的发酵培养基优化[J]. 食品与发酵工业, 2010, 36(03): 108-111,116.
Li Wenjing,Zhao Xiangying,Tian Yanjun,et al. Optimization of γ-PGA Fermentation Medium of Bacillus amyloliquefaciens by Response Surface Methodology[J]. Food and Fermentation Industries, 2010, 36(03): 108-111,116.