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食品与发酵工业  2021, Vol. 47 Issue (7): 27-33    DOI: 10.13995/j.cnki.11-1802/ts.025732
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
静电喷雾干燥微囊化乳双歧杆菌BL03
姜甜1, 陆文伟1,2, 崔树茂1, 张灏1,2,3, 赵建新1,2*
1(江南大学 食品学院,江苏 无锡, 214122)
2(江南大学(扬州)食品生物技术研究所,江苏 扬州, 225004)
3(江南大学国家功能食品工程技术研究中心,江苏 无锡, 214122)
Electrostatic spray drying microencapsulated Bifidobacterium lactis BL03
JIANG Tian1, LU Wenwei1,2, CUI Shumao1, ZHANG Hao1,2,3, ZHAO Jianxin1,2*
1(College of Food,Jiangnan University,Wuxi 214122,China)
2(Institute of Food Biotechnology,Jiangnan University(Yangzhou),Yangzhou 225004,China)
3(National Engineering Research Center for Functional Food,Jiangnan University,Wuxi 214122,China)
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摘要 静电喷雾干燥是新型微囊化益生菌的方法。该文研究了静电喷雾干燥乳双歧杆菌BL03保护剂和微囊化工艺,通过单因素和正交试验,优化得到保护剂组成为海藻糖14%,亚麻酸10%,CaHPO4 6%,β-环糊精11%(均为质量分数),最佳静电喷雾微囊化工艺进风温度80 ℃,物料泵流量30 r/min,静电压25 kV,存活率高达89.26%。乳双歧杆菌BL03菌粉扫描电镜图片显示,静电喷雾干燥微囊化包埋效果最好,包埋率高达93.3%。比较静电喷雾干燥、常规干燥和乳化冷冻干燥3种微囊化方法,静电喷雾干燥模拟胃液作用2 h,存活率超过80%;模拟肠液作用4 h,存活率达到75%;25 ℃下储存12个月,活菌数存活率超过70%,均明显优于常规干燥和乳化冷冻干燥,说明静电喷雾干燥微囊化方法适合包埋乳双歧杆菌。
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姜甜
陆文伟
崔树茂
张灏
赵建新
关键词:  静电喷雾干燥  乳双歧杆菌  益生菌  微囊化    
Abstract: Electrostatic spray drying is a new microencapsulated probiotics method.The protectants and process of electrostatic spray drying were determined using single factor design and orthogonal experiment for Bifidobacterium lactis BL03.The optimized protectants were 14% trehalose, 10% linolenic acid, 6% calcium hydrophosphate and 11% β-cyclodextrin.The optimized process for electrostatic spray drying microencapsules were as follows:inlet temperature was 80 ℃, pump flow was 30 r/min, electrostatic charge was 25 kV and the survival rate was 89.26%.Scanning electron microscope (SEM) images showed that electrostatic spray drying had the best effect of microencapsulation.Embedding rate of microencapsules was 93.3% for B.lactis BL03.Compared electrostatic spray drying with traditional spray drying and freeze drying, the survival rate of electrostatic spray drying was above 80% when treated with simulated gastric fluid for 2 hours, and reached 75% with simulated intestinal fluid after 4 hours. The survival rate was over 70% at the temperature of 25 ℃ after 12 months.Electrostatic spray drying was superior to traditional spray drying and freeze drying.
Key words:  electrostatic spray drying    Bifidobacterium lactis    probiotics    microencapsulation
收稿日期:  2020-09-30      修回日期:  2020-11-07           出版日期:  2021-04-15      发布日期:  2021-05-20      期的出版日期:  2021-04-15
基金资助: 国家食品科学与工程一流学科建设项目(JUFSTR20180102)
作者简介:  博士研究生(赵建新教授为通讯作者,E-mail:zhaojianxin@jiangnan.edu.cn)
引用本文:    
姜甜,陆文伟,崔树茂,等. 静电喷雾干燥微囊化乳双歧杆菌BL03[J]. 食品与发酵工业, 2021, 47(7): 27-33.
JIANG Tian,LU Wenwei,CUI Shumao,et al. Electrostatic spray drying microencapsulated Bifidobacterium lactis BL03[J]. Food and Fermentation Industries, 2021, 47(7): 27-33.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.025732  或          http://sf1970.cnif.cn/CN/Y2021/V47/I7/27
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