Abstract: To enhance the detection sensitivity of plant endogenous sugars and sugar alcohols, the quantitative analysis of multi-components by gas chromatography-tandem mass spectrometry (GC-MS/MS) for the determination of glucose, galactose, inositol, mannitol, sorbitol and sucrose was established by using adonitol as an internal standard substance. The sample was extracted by organic solvent and then mixed with pyridine, hydroxylamine hydrochloride and acetic anhydride for derivatization. The gas chromatography separation was performed on an HP-5MS capillary column. And the acetylated sugar and sugar alcohols were determined by MS in multiple reaction monitoring(MRM)mode. The results showed that, in the acquisition mode of mass spectrometry, there was a good linear relationship between the concentration of 6 acetylated sugars and sugar alcohols and detection response which with a correlation coefficients(R2) greater than 0.997. The detection limits (LODs) and quantification(LOQs) were 0.013-0.12 mg/L and 0.045-0.37 mg/L, respectively. The sample recovery rates ranged from 95.38％-102.6％, and the relative standard deviations were 1.28％-3.42％.The method has the advantages of low sample dosage, high accuracy, good precision, and thus could be successfully applied to the analysis of sugars and sugar alcohols in plant tissues at a low concentration.
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2021, Vol. 47 
