可发酵三七等中药材的食用菌种筛选和皂苷生物转化产物的分析 

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  • 1(北京中医药大学  中药学院,北京,10029)2(江南大学  生物工程学院,江苏  无锡,214063) 3(中国食品发酵工业研究院,北京,10027)

网络出版日期: 2018-01-08

Screening of edible fungi for fermentation of Panax notoginseng Chinese medicinal herbs and analysis of#br# biotransformation products of saponins #br#

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  • 1(BeijingUniversityofChineseMedicine,Beijing,10029)2(ScholofBiotechnology,JiangsuUniversity,Wuxi21412 ,China) 3(ChinaNationalResearchInstituteofFodandFermentationIndustries,Beijing10027,China)

Online published: 2018-01-08

摘要

研究了保加利亚乳杆菌、凝结芽孢杆菌、鼠李糖乳杆菌和冠突散囊菌等食品工业中常用的微生物菌种对三七之类的中药原料的生物转化,利用 HPLC分析了不同菌种直接发酵对人参皂苷组成的影响。通过不同微生物菌种的发酵,三七、人参和西洋参所含的 Rb1、Re等常见人参皂苷会分别转化为 Rh1、CK和 Rd等稀有人参皂苷,产物中没有检测到 Rh2等人参皂苷,说明菌种产生的糖苷酶具有相当的专一性,为进一步对特殊目标皂苷的开发利用提供了基础。

关键词: 三七; 皂苷; 生物转化

本文引用格式

李粟琳, 张翔宇, 王洋, 等 . 可发酵三七等中药材的食用菌种筛选和皂苷生物转化产物的分析 [J]. 食品与发酵工业, 2017 , 43(12) : 164 -168 . DOI: 10.13995/j.cnki.11-1802/ts.014158

Abstract

hecultivationandaplicationofthemedicinalplantsofPanaxginsenginChina,includingPanax notoginsenghasalonghistoryanditisofgreatsignificancetothedevelopmentofthesepreciousresourcesbyfermen tation.Inthispaper,westudiedthefermentationprocesesoftheserawmaterialswithmicrobialstrainsbelongtoLac tobacilusbulgaricus,Baciluscoagulans,LactobacilusrhamnosusandEurotium cristatum etc.whichcommonlyused infod industry. HPLC wasused toanalyzethechangesofginsenosidecompositon afterdiferentfermentaion. Throughthefermentationwithdiferentmicrobialstrains,Rb1,Reandothercommonginsenosidewhichcontainedin Panaxnotoginseng,ginsengandAmericanginsengwerebiotransformedrespectivelyintoRh1,CK andRd,while ginsenosideRh2wasnotdetectedintheproduct.Theresultssugestedthattheglycosidasesfrom thesestrainswere veryspecificandprovidedabasisforthebiotransformationandutilizationofspecialtargetsaponins.
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