透射电镜技术可观察到小于0.2μm的细胞的超微结构，从而可在分子层面对果蔬样品进行更深入的研究。本文介绍了透射电镜技术的工作原理和发展史，综合论述了透射电镜技术在确定果蔬的最佳采摘期、采后贮藏条件以及选择适宜的保鲜方法等方面的应用现状，最后对透射电镜技术未来在果蔬保鲜及相关研究的应用前景进行了展望，以期透射电镜技术可以在果蔬保鲜方面能得到更广泛的应用。

Transmission electron microscopy (TEM)  can observe   ultrastructure of less than 0.2μm cell, consequently this technology can conduct the more in-depth research of the fruit and vegetables amples at the molecular level. The paper introduces the working principle and the history of transmission electron microscopy (TEM) technology, and also comprehensively discusses the experimental conditions the best harvest of fruit, the postharvest storage condition and appropriate preservation methods determined by TEM technology. The application prospect of TEM technology used in fresh-keeping and related research is also discussed. TEM technology will get more extensive application in fruits and vegetables preservation.