Lactobacillus quorum sensing system is an important
regulatory pathway for lactic acid bacteria to respond to environmental changes
and regulate the expression of related genes in vivo.In order to study the
quorum sensing system of lactic acid bacteria,In this study, AI-2 was detected
in 8 strains of lactic acid bacteria, and 7 strains of lactic acid bacteria
were detected in the AI-2 signal molecules, and 3 of them produce AI-2 signal
is very strong.For strains with high yield AI-2,the expression ofluxSgene andLDHgene of lactic acid bacteria were detected by RT-PCR. The
expression of luxS gene was higher in lactic acid bacteria with high yield of
AI-2, and the expression ofLDHgene
was weaker.Finally, the correlation between acid environment and AI-2
production of lactic acid bacteria was analyzed. The results showed that the
stronger the acidity of the culture medium, the stronger the AI-2 signal.LuxS
gene is the key gene of AI-2 signal synthesis. AI-2 signal molecule improves
the acid resistance of lactic acid bacteria, which is beneficial to the lactic
acid bacteria to regulate the acid-base balance and flora balance in the
intestine through the group induction signal.This study lays the foundation for
actic acid bacteria population induction signaling pathway, to explore the
lactic acid bacteria in the host intestinal adhesion colonization the mechanism
of subsequent analysis.
