群体感应信号分子 AI-2 高产乳酸菌株筛选及特性研究

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  • 1.(山西师范大学生命科学学院,山西 临汾,041000) 2.(中国农业科学院农产品加工研究所,北京,100193)
蔡针华,硕士研究生,主要从事生物化学与分子生物学研究。E-mail:1135887646 @qq.com。

网络出版日期: 2018-02-02

基金资助

国家自然科学基金面上项目(31471603);国家科技支撑计划项目(2015BAD16B00);中国农业科学院科技创新工程项目(cxgc-ifst-08)

Screening and characterization research of quorum sensing signaling molecule AI-2 high-yield Lactobacillus strains

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  • 1.(School of Life Science,Shanxi Normal University,Linfen ,041000,China) 2.(Institute of food science and technology CAAS,Beijing ,100193,China)

Online published: 2018-02-02

摘要

乳酸菌群体感应系统是乳酸菌响应环境变化、调节体内相关基因表达的重要调控通路。为深入研究乳酸菌的群体感应系统,对8株乳酸菌产群体感应信号分子自体诱导剂-2(Autoinducer-2,AI-2)情况进行了检测,7株乳酸菌中检测到明显的AI-2信号分子,且其中3株产生AI-2信号极强;对高产AI-2的菌株,通过RT-PCR对乳酸菌luxS基因和LDH基因的表达情况进行了检测,发现高产AI-2的乳酸菌其luxS基因表达较强,而LDH基因表达较弱;最后分析了酸性环境与乳酸菌信号分子AI-2产量的相关性,结果表明培养基酸性越强,乳酸菌产AI-2信号越强。luxS基因是AI-2信号合成的关键基因,AI-2信号分子提高了乳酸菌的耐酸性,有利于乳酸菌通过群体感应信号调控肠道内的酸碱平衡和菌群平衡。本研究为后续解析乳酸菌群体感应信号传导通路、探索乳酸菌在宿主肠道内粘附定植的机制奠定基础。

本文引用格式

蔡针华, 程娜, 贾震虎, 等 . 群体感应信号分子 AI-2 高产乳酸菌株筛选及特性研究[J]. 食品与发酵工业, 2018 , 44(1) : 66 . DOI: 10.13995/j.cnki.11-1802/ts.015617

Abstract

Lactobacillus quorum sensing system is an important regulatory pathway for lactic acid bacteria to respond to environmental changes and regulate the expression of related genes in vivo.In order to study the quorum sensing system of lactic acid bacteria,In this study, AI-2 was detected in 8 strains of lactic acid bacteria, and 7 strains of lactic acid bacteria were detected in the AI-2 signal molecules, and 3 of them produce AI-2 signal is very strong.For strains with high yield AI-2,the expression ofluxSgene andLDHgene of lactic acid bacteria were detected by RT-PCR. The expression of luxS gene was higher in lactic acid bacteria with high yield of AI-2, and the expression ofLDHgene was weaker.Finally, the correlation between acid environment and AI-2 production of lactic acid bacteria was analyzed. The results showed that the stronger the acidity of the culture medium, the stronger the AI-2 signal.LuxS gene is the key gene of AI-2 signal synthesis. AI-2 signal molecule improves the acid resistance of lactic acid bacteria, which is beneficial to the lactic acid bacteria to regulate the acid-base balance and flora balance in the intestine through the group induction signal.This study lays the foundation for actic acid bacteria population induction signaling pathway, to explore the lactic acid bacteria in the host intestinal adhesion colonization the mechanism of subsequent analysis.
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