新疆风干肉中优势乳酸菌的分离及产酶特性分析

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  • 1(新疆农垦科学院  农产品加工研究所,新疆  石河子,832000)2(新疆农垦科学院农产品加工重点实验室,新疆  石河子,832000)
李宇辉,硕士,助理研究员,研究方向为畜产品加工,E-mail:liyuhui615@sina.com

网络出版日期: 2018-02-02

基金资助

新疆生产建设兵团工业科技攻关与成果转化项目(2015AB023);新疆农垦科学院青年基金项目(YQJ201506)

Isolation of dominant lactic acid bacteria from Xinjiang dried meat and analysis of its enzyme-producing characteristics

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  • 1.(Institute of agro-products processing science and technology Xinjiang academy of agricultural and reclamation science, Shihezi, 832000,China) 2. (Key Laboratory of Agro-Products Processing,XinJiangAcademyof Agricultural And Reclamation Science, Shihezi, 832000,China)

Online published: 2018-02-02

摘要

对新疆风干肉中优势乳酸菌进行筛选,并对其产酶特性进行了分析。利用可培养方法,对新疆风干肉中乳酸菌进行分离筛选,结合生理生化和16SrRNA序列对其鉴定,并分析其在不同温度下的产酶特性。结果显示:菌株tL-4为植物乳杆菌(Lactobacillus plantarum);tL-7为棒状乳杆菌(Lactobacillus coryniformis),而菌株aL-16为戊糖乳杆菌(Lactobacillus pentosus)。3 株菌的乳酸脱氢酶活性都较高,相比而言,tL-4 和aL-16 比tL-7 有较高活性,分别为112.86 U/mL 和94.62 U/mL。aL-16的亚硝酸盐还原酶活力最高,为14.72 U/mL,tL-7的亚硝酸盐还原酶活力较低,4.63 U/mL。菌株tL-7的酯酶活力较高为13.25 U/mL,tL-4的酯酶活力为11.45 U/mL,而aL-16的酯酶活力相对较低9.67 U/mL。3株乳酸菌在不同的温度下的产酶特性也不同,且菌株间的最适产酶温度存在差异,3株菌温度在30-40℃产酶能力强,超出这个温度范围,产酶能力下降。

本文引用格式

李宇辉 , 郭安民, 刘成江, 等 . 新疆风干肉中优势乳酸菌的分离及产酶特性分析[J]. 食品与发酵工业, 2018 , 44(1) : 80 . DOI: 10.13995/j.cnki.11-1802/ts.015523

Abstract

The dominant lactic acid bacteria were screened in Xinjiang Air-dried meat,and the enzyme production characteristics were alsostydied. Using culture methods, lactic acid bacteria were isolated and screened from Xingjiang Air-dried meat. The isolates were identified by standard physiological and biochemical tests and analysis of 16SrRNA gene sequencing technique. Studied the enzyme production capacity on the different temperature. The results showed that three isolation strain were Lactobacillus plantarum (tL-4), Lactobacillus coryniformis (tL-7), and Lactobacillus pentosus (aL-16). tL-4 and aL-16 showed relatively higher lactic dehydrogenase activity than tL-7.The values reached 112.86.34 U/mL and 94.62 U/mL, respectively. aL-16 presented relatively high nitrite reductase activity. The values reached 14.72 U/mL, respectively. Lactobacillus coryniformis possessed the lowest nitrite reductase activity (4.63U/mL). Compared with the aL-16, tL-7and tL-4 displayed higher esterase activity. The values reached 13.25 U/mL and 11.45U/mL, respectively. Inter-strain differences were presented in the optimal enzyme-producing temperature among the three strains and the optimum conditions for the same strain to produce various enzymes were different. The optimum temperatures for the isolated LAB strains to produce various enzymes were 30 ℃-40℃ respectively. Beyond this temperature range, the enzyme producing ability became weaker.
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