对新疆风干肉中优势乳酸菌进行筛选,并对其产酶特性进行了分析。利用可培养方法,对新疆风干肉中乳酸菌进行分离筛选,结合生理生化和16SrRNA序列对其鉴定,并分析其在不同温度下的产酶特性。结果显示:菌株tL-4为植物乳杆菌(Lactobacillus plantarum);tL-7为棒状乳杆菌(Lactobacillus coryniformis),而菌株aL-16为戊糖乳杆菌(Lactobacillus pentosus)。3 株菌的乳酸脱氢酶活性都较高,相比而言,tL-4
和aL-16 比tL-7 有较高活性,分别为112.86
U/mL 和94.62 U/mL。aL-16的亚硝酸盐还原酶活力最高,为14.72
U/mL,tL-7的亚硝酸盐还原酶活力较低,4.63 U/mL。菌株tL-7的酯酶活力较高为13.25
U/mL,tL-4的酯酶活力为11.45 U/mL,而aL-16的酯酶活力相对较低9.67
U/mL。3株乳酸菌在不同的温度下的产酶特性也不同,且菌株间的最适产酶温度存在差异,3株菌温度在30-40℃产酶能力强,超出这个温度范围,产酶能力下降。
The dominant lactic acid
bacteria were screened in Xinjiang Air-dried meat,and the enzyme production characteristics were
alsostydied. Using culture methods, lactic acid bacteria were isolated and
screened from Xingjiang Air-dried meat.
The isolates were identified by standard physiological and biochemical tests
and analysis of 16SrRNA gene sequencing technique. Studied the enzyme
production capacity on the different temperature. The results showed
that three isolation strain were Lactobacillus plantarum (tL-4), Lactobacillus
coryniformis (tL-7), and Lactobacillus pentosus (aL-16). tL-4 and aL-16 showed
relatively higher lactic dehydrogenase activity than tL-7.The values reached
112.86.34 U/mL and 94.62 U/mL, respectively. aL-16 presented relatively high nitrite
reductase activity. The values reached 14.72 U/mL, respectively. Lactobacillus
coryniformis possessed the lowest nitrite reductase activity (4.63U/mL).
Compared with the aL-16, tL-7and tL-4 displayed higher esterase activity. The
values reached 13.25 U/mL and 11.45U/mL, respectively. Inter-strain differences
were presented in the optimal enzyme-producing temperature among the three
strains and the optimum conditions for the same strain to produce various
enzymes were different. The optimum temperatures for the isolated LAB strains
to produce various enzymes were 30 ℃-40℃ respectively. Beyond this temperature
range, the enzyme producing ability became weaker.