“肽植”固体饮料的体外活性和对斑马鱼肝脏保护作用的评价

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  • 1(宁波御坊堂生物科技有限公司,浙江宁波315012) 2(中国食品发酵工业研究院,北京朝阳100015)
贾福怀,学士,高级工程师,研究方向为健康产品研发。E-mail:jfh9625@163.com

网络出版日期: 2018-02-02

基金资助

国家重点基础研究发展计划(973计划)(2016YFD0401303);北京市科技计划项目(D171100001917003)

The evaluations of in vitro activity and zebrafi sh liver protection by “Taizhi” solid drink

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  • 1 (Ningbo Yufangtang Biological Technology Co. Ltd., Zhejiang Ningbo, 315012) 2 (China National Research Institute of Food & Fermentation Industries, Beijing Chaoyang, 100015)

Online published: 2018-02-02

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摘要

主要研究了以药食两用中药和玉米低聚肽为基础原料而开发的一款固体饮料的体外活性评价和护肝功能特性。分别采用还原Fe3+和1,1-二苯基-2-三硝基苯肼(DPPH)法测定了该固体饮料的总还原力和DPPH·自由基的清除率,并且通过HepG2细胞脂肪变性模型考察了其促进甘油三酯代谢的能力,最后通过斑马鱼实验观察该产品对斑马鱼肝损伤保护作用。实验表明该产品具有很好的抗氧化能力,具有明显的调控脂质代谢的作用,同时对对乙酰氨基酚诱发的斑马鱼肝损伤有保护作用

本文引用格式

贾福怀, 韩晓峰, 李志军, 等 . “肽植”固体饮料的体外活性和对斑马鱼肝脏保护作用的评价[J]. 食品与发酵工业, 2018 , 44(1) : 98 . DOI: 10.13995/j.cnki.11-1802/ts.014782

Abstract

ObjectiveThis paper mainly studied the in vitro activity and the zebrafish liver protection of the “Taizhi” solid drink, which is developed based on edible Chinese herbals and corn oligopeptide.MethodThe antioxidant activity of this solid drink was determined by Fe3+reducing power and DPPH free radical scavenging ability. The total triglyceride (TG) was used to evaluate the effect of the solid drink in free fatty acids (Oleic acid and palmitic acid)-induced steatosis in HepG2 cells model. The liver protection was investigated via the zebrafish model of liver injury induced by acetaminophen.ConclusionThe results showed that the “Taizhi” solid drink had excellent ability of antioxidation, obvious modulation effect of lipid metabolism, and the function of liver protection on the zebrafish model of liver injury induced by acetaminophen.
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