在摇瓶和发酵罐上研究了分批补料发酵对枯草芽孢杆菌LSSE-22发酵生产纳豆激酶的影响。通过摇瓶分批发酵，确定最优碳源和氮源分别为葡萄糖和大豆蛋白胨。在优化初始葡萄糖和大豆蛋白胨浓度的基础上，进一步研究了补料底物、补料方式和补料时间对产酶的影响。结果表明，采用分批补糖发酵工艺, 纳豆激酶产量可达到1437.34 IU/mL，比分批培养提高了21.38%。在7.5 L发酵罐上进行分批补料发酵放大实验，纳豆激酶产量可达2046.47 IU/mL，明显优于分批培养。

The effect of fed-batch fermentation on the production of nattokinase byBacillus subtilisLSSE-22 was investigated in shake flask and bioreactor. Glucose and soy peptone were identified as the optimal carbon source and nitrogen source through batch fermentation in shake flask, respectively. Furthermore, the effect of feeding substrate, feeding manner and feeding time on the production of nattokinase was studied on the basis of optimization of initial concentration of glucose and soy peptone. The results showed that the production of nattokinase was 1437.34 IU/mL, which was 21.38% higher than that by batch culture. In the 7.5 L bioreactor, the maximal production of nattokinase reached 2046.47 IU/mL, which was significantly higher than that by batch culture.