响应面优化的南极磷虾蛋白磷酸化改性工艺

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  • (1.上海理工大学医疗器械与食品学院,中国上海 200093) (2.中国水产科学研究院东海水产研究所,中国上海 200090) (3.上海海洋大学食品学院,中国上海 201306) (4.上海市农业科学院食用菌研究所,中国上海 201403)
戚亭(1990-),女,安徽淮北人,在读硕士研究生,从事食品生物技术研究,zdliu1976@163.com;

网络出版日期: 2018-02-05

基金资助

中央级公益性科研院所基本科研业务费专项资金 (2016HY-ZD0903;2016HY-ZD1003) ;上海市科技兴农项目(沪农科攻字(2015)第5-5号);公益性行业(农业)科研专项(201203018);中央级公益性科研院所基本科研业务费专项资金 (2011M06)。

Phosphorylation process of Antarctic krill protein with response surface optimization

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  • (1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China) (2. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences. Shanghai 200090, China) (3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China) (4. Institute of edible fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

Online published: 2018-02-05

摘要

为进一步改善南极磷虾蛋白的加工特性,采用三聚磷酸钠对南极磷虾蛋白进行磷酸化改性,利用傅里叶红外光谱技术考察南极磷虾蛋白磷酸化实施的可能性;以磷酸化程度为考核指标,考查蛋白浓度、三聚磷酸钠添加量、pH值等因素对南极磷虾蛋白磷酸化程度的影响;通过响应面设计法优化获得南极磷虾蛋白磷酸化改性的最佳工艺条件为:蛋白浓度22g/L、三聚磷酸钠添加量6.55g/100g蛋白、反应pH值9.04、反应时间1.55h、反应温度39.90?℃,在该条件下磷酸化水平41.91mg/g。实验结果表明,采用三聚磷酸钠对南极磷虾蛋白的磷酸化改性是切实可行的。

本文引用格式

戚亭, 陈雪忠, 刘志东, 等 . 响应面优化的南极磷虾蛋白磷酸化改性工艺[J]. 食品与发酵工业, 2018 , 44(1) : 204 . DOI: 10.13995/j.cnki.11-1802/ts.014638

Abstract

In order to further improve Antarctic krill protein processing characteristics, Antarctic krill protein was modified by sodium tripolyphosphate. Firstly, whether the Antarctic krill protein would be phosphorylated was investigated by FT-IR. Then, taking the phosphorylation degree as index, the optimum reaction parameters of Antarctic krill protein phosphorylation were determined by the single and orthogonal experiments. The result suggested that the optimum conditions were protein concentration 22g/L,STP addition of 6.55g/100g蛋白,heating time 1.55?h,pH?9.04,temperature 39.90?℃.Under these conditions, the phosphorylation degree was 41.91?mg/g. Theresult indicate that the use of sodium tripolyphosphate on the Antarctic krill protein phosphorylation is feasible.
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