Encapsulated enzymes by
using yeast spores is a new enzyme-immobilized technology. It was reported that
the activity of encapsulated enzymes was largely improved bySaccharomyces cerevisiaespores ofosw2Δwhich has mild spore wall
defective and theosw2Δspore-encapsulate
enzymes also exhibit resistance to environmental stresses and have a good
reusability. To investigate whetherkre1Δspores have similar ability, the creatininase gene was transformed into yeast cells and the properties of encapsulated
enzymes were examined. The results suggested the activity of the creatininase
encapsulated inkre1Δspores was
similar toosw2Δ
spores but was
significantly higher than that of wild type spores. Compared with free enzymes,
likeosw2Δ,encapsulated creatininase in
kre1Δspores exhibits resistance to environmental stresses and has a good reusability. The
spore-encapsulated creatininase inkre1Δandosw2Δshows highest activity at 60oC,
pH 6.0. Moreover, thekre1Δspore-encapsulated
enzymes are resistant to high temperature and exhibit a wide range of pH
tolerance as same as that ofosw2Δspores. Therefore,kre1Δspores can
be used as a
superior carrier of enzyme capsules.