he expression of several metabolic behaviors of bacteria including biofilm formation and swimming motility, which can cause food spoilage and foodborne desease, is generally regulated by quorum sensing (QS), so it makes sense to analyze the effects of environmental factors on bacterial QS for the quality and safety control of fish products.In the study, bioreporter (Agrobacterium tumefaeiensA136) and confocal laser scanning microscope (CLSM) were applied to observe the effects of different pH (6.0, 7.0, 8.0) and NaCl concentration (0.5%, 1.0%, 2.0%) onN-acyl homoserine lactone (AHL) type QS and metabolic behaviors ofAeromonasveroniibv.veroniiisolated from fermented surimi.The results showed that the initial pH of 8.0 and NaCl of 2.0% could inhibit the AHL production, biofilm formation and swimming motility (P< 0.05). It was suggested thatpH and NaCl concentration could affect the AHL production and the expression of bacterial QS and thus affect the regulation of bacterial behaviors by QS.