以雌性中华绒螯蟹关键脂质为研究对象,基于挥发性成分分析,从时间、温度、水分、氧气4个热处理环境因素出发,搭建热氧化体外模型并进行条件优化,为脂质氧化降解特性的研究提供理论基础。采用新型吸附材料(mono trap)作为顶空固相萃取整体捕集剂,结合气相色谱-质谱仪(GC-MS)对雌蟹性腺和肝胰腺在热处理过程中的挥发性物质进行测定,优化热处理时间和温度;在此基础上采用电子鼻技术结合主成分和雷达图分析法分别对性腺和肝胰腺中的关键脂质,甘油三酯(triglyceride, TG)和脑磷脂(phosphatidyl ethanolamine, PE)的水分和氧气进行优化。结果显示,最优热氧化体系条件分别为,TG: 100 ℃, 25 min,水分6 μL,有氧条件;PE: 100 ℃,25 min,水分0 μL,有氧条件。
The thermal oxidation system (conditions: temperature, time, water, oxygen) of key lipids in female Eriocheir sinensis was established based on volatile compound analysis. The establishment of vitro model can provide a reaction condition for the research of oxidation degradation characteristics of lipids. The new adsorption material-Mono Trap, combined with gas chromatography mass spectrometry (GC-MS) was used to extract volatile compounds during the heat treatment of female crab gonads and hepatopancreas. Furthermore, electronic nose combined with principle components analysis (PCA) and radar picture analysis was used to optimize water and oxygen of Triglyceride (TG) and Phosphatidyl ethanolamine (PE). The results indicated that the optimal condition of thermal oxidation for TG and PE were: TG: 100 ℃, 25 min, 6 μL water, aerobic conditions; PE: 100 ℃, 25 min, 0 μL water, aerobic conditions.
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